Broad spectrum antibiotic peptide
AssigneeKansas State University Research Foundation
InventorsJohn J. Iandolo, Scott Crupper
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Abstract
Improved antimicrobial proteinaceous substances produced by Staphylococcus aureus KSI1829 (e.g., Bac1829) and methods of inhibiting microbial growth using such substances are disclosed.
Claims31
An at least partially purified antimicrobial proteinaceous substance produced by Staphyloccocus aureus having a molecular weight of from about 6 to 7 kilodaltons, wherein said substance at a concentration of 640 antimicrobial units/ml in an aqueous solution retains essentially all of its antimicrobial activity after one or more of the following treatments:
- (1) heat treatment at 95.degree. C. for 15 minutes, (2) treatment with 6M urea or 10 mM dithiothreitol at room temperature for 1 hour, and (3) treatment with deoxyribonuclease, ribonuclease, or lysostaphin at a concentration of 1 mg/ml at room temperature for 1 hour.
A method of inhibiting microbial growth in an environment capable of sustaining said growth comprising administering to said environment a microbial growth-inhibiting amount of an at least partially purified antimicrobial proteinaceous substance produced by Staphylococcus aureus having a molecular weight of from about 6 to 7 kilodaltons, wherein said substance at a concentration of 640 antimicrobial units/ml in an aqueous solution retains essentially all of its antimicrobial activity after one or more of the following treatment:
- (1) heat treatment at 95.degree. C. for 15 minutes, (2) treatment with 6M urea or 10 mM dithiothreitol at room temperature for 1 hour, and (3) treatment with deoxyribonuclease, ribonuclease, or lysostaphin at a concentration of 1 mg/ml at room temperature for 1 hour.
Dependencies are inferred from the claim text.