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US 5,702,955 AGranted
Ascorbate resistant detection of hydrogen peroxide
AssigneeBayer Aktiengesellschaft
InventorMichael Pugia
Life of this patent
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Abstract
The present invention is a method for the detection of hydrogen peroxide in biological fluids or an aqueous solution which involves contacting the solution with an oxidation-reduction indicator and a transition metal complex. The transition metal complex is either a creatinine coordinated with iron or a guanidine coordinated with iron.
Claims25
A method for the detection of hydrogen peroxide in a solution which may contain ascorbate, comprising the steps of:
- (a) adding to said solution an oxidation-reduction indicator and a transition metal complex having the structure:
- ##STR5## wherein X and Y are independently chosen from the group consisting of creatinine, chloride, fluoride, bromide, iodide, sulfate, phosphate, perchlorate, nitrate, oxalate, sulfide, ammonium, gluconate, cyanide, thiocyanate, catechol, tropolone, phenol, pyridine, acetate, citrate, tartaric acid, malonic acid, boric acid, succinic acid, glycerol-2-phosphate, salicylic acid, oxalic acid, and malic acid;
- and
- (b) analyzing the color transition to detect the presence or concentration of hydrogen peroxide.
1 more element
A method for the detection of hydrogen peroxide in a solution which may contain ascorbate, comprising the steps of:
- (a) adding to said solution an oxidation-reduction indicator and a transition metal complex having the structure:
- ##STR6## wherein X and Y are independently chosen from the group consisting of creatinine, chloride, fluoride, bromide, iodide, sulfate, phosphate, perchlorate, nitrate, oxalate, sulfide, ammonium, gluconate, cyanide, thiocyanate, catechol, tropolone, phenol, pyridine, acetate, citrate, tartaric acid, malonic acid, boric acid, succinic acid, glycerol-2-phosphate, salicylic acid, oxalic acid, and malic acid, and R.sub.1 and R.sub.2 are independently chosen from the group consisting of C.sub.n H.sub.2n+1, (C.sub.2 H.sub.4 O).sub.n H, C.sub.5 H.sub.4 Z, C.sub.4 H.sub.3 Z, and C.sub.11 H.sub.6 Z, wherein n is an integer from 1 to 20, and Z is O, S, N, or CH;
- and
- (b) analyzing the color transition to detect the presence or concentration of hydrogen peroxide.
1 more element
Dependencies are inferred from the claim text.