US 5,702,944 A · Microbial transport media

Abstract

A microbial transport medium for the collection, transport and storage of samples suspected of having Chlamydia, Mycoplasma, Ureaplasma or viral pathogens comprises a balanced salt solution, a proteinaceous stabilizer, and carbohydrate and amino acid nutrient sources. The medium is buffered to maintain physiological pH and includes a pH indicator in order to indicate variation of pH outside the physiological pH range. The medium further comprises antimicrobial and antifungal agents and can include gelatin. Samples can be stored in the medium at temperatures ranging from room temperature to minus 70.degree. C. Additionally, the transport medium can be used in standardized commercial ELISA and PCR assays.

Claims4

Dependent claims

A aqueous transport medium capable of maintaining the viability of a plurality of microorganisms selected from the group consisting of Chlamydia, Mycoplasma, Ureaplasma, and viruses, said medium having physiological pH and comprising about 94-96.75 ml of water and:

______________________________________ gelatin (Bloom value 40-125) 0-20.0 g sugar 65-75 g HEPES 5-7 g KCl 0.3-0.6 g L-glutamic acid 0.5-1.0 g phenol red 5-15 mg CaCl.sub.2 0.1-0.5 g MgSO.sub.4.7H.sub.2 O 0.1-0.3 g bovine serum albumin 1.0-20.0 g vancomycin 0.01-0.05 g colistin 100,000-250,000 units amphotericin B 0.5-3.0 mg. ______________________________________

Dependencies are inferred from the claim text.

Microbial transport media

Life of this patent

Microbial transport media

Life of this patent

Abstract

Claims4