Glucosamine-6-phosphate deaminase and process for producing the same
AssigneeAgency of Industrial Science and Technology
InventorsShizu Fujishima, Naoko Yamano
Life of this patent
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Abstract
A glucosamine 6-phosphate deaminase having specific physicochemical properties. A process for producing the glucosamine 6-phosphate deaminase which comprises incubating a microorganism belonging to the genus Vibrio and harvesting the glucosamine 6-phosphate deaminase from the culture thus obtained.
Claims3
An isolated and purified glucosamine-6-phosphate deaminase which is produced by a microorganism belonging to the genus Vibrio and identified on the basis of the following physicochemical properties:
- (1) function:
- acting on the amino group of D-glucosamine 6-phosphate to thereby form D-fructose 6-phosphate;
- (2) substrate specificity:
- acting on D-glucosamine 6-phosphate but not on D-glucosamine;
- (3) optimum pH value:
- 7.4 to 7.8;
- (4) stable pH value:
- 7.0 to 9.0 (after incubating at 37.degree. C. for 30 minutes, at least 70% of the activity is sustained);
- (5) optimum temperature:
- 37.degree. to 38.degree. C;
- and
- (6) heat stability:
- 50.degree. C. (after incubating at pH 7.5 for 30 minutes, at least 70% of the activity is sustained).
A process for producing a glucosamine-6-phosphate deaminase which comprises incubating a microorganism belonging to the genus Vibrio and capable of producing a glucosamine-6-phosphate deaminase and harvesting the glucosamine-6-phosphate deaminase from the culture thus obtained.
Dependencies are inferred from the claim text.