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US 5,702,933 AGranted
Control of fruit ripening and senescence in plants
AssigneeMonsanto Company
InventorsHarry John Klee, Ganesh Murthy Kishore
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AssigneeMonsanto Company
InventorsHarry John Klee, Ganesh Murthy Kishore
Estimating the term…
A method for controlling the ripening of fruits and vegetables as well as a method for controlling senescence of plant tissue is described. The method generally embraces the expression of an ACC metabolizing enzyme in the fruit or other desired plant tissue to inhibit the production of ethylene in the fruit or plant tissue. The use of the ACC metabolizing enzyme ACC deaminase is described in detail. The ripening or senescence process in the fruit or plant tissue is inhibited by the expression of the ACC deaminase gene such that the shelf-life and marketability of the fruit or plant is enhanced. The ACC metabolizing enzyme may be used in combination with other methods for reducing ethylene production in transformed plants to further reduce the production of ethylene in the fruit or plant. DNA constructs containing the ACC deaminase gene are also described.
A recombinant, double stranded DNA molecule comprising in sequence in the 5' to 3' direction:
A method for producing fruit-bearing plants, wherein the fruit exhibit a delayed ripening phenotype, said method comprising the steps of:
A method for producing fruit-bearing plants wherein said fruit exhibits a delayed ripening phenotype comprising expressing a gene encoding 1-aminocyclopropane-1-carboxylic acid deaminase in said plant at a level sufficient to reduce the production of ethylene in said fruit.
A transformed plant which exhibits a delayed ripening phenotype, said plant containing in its genome a recombinant, double-stranded DNA molecule, said molecule comprising in sequence in the 5' to 3' direction:
A method for producing a plant with reduced levels of ethylene which comprises expressing a gene encoding a 1-aminocyclopropane-1-carboxylic acid metabolizing enzyme in said plant at a sufficient level to reduce the steady-state 1-aminocyclopropane-1-carboxylic acid concentration, wherein said 1-aminocyclopropane-1-carboxylic acid metabolizing enzyme is 1-aminocyclopropane-1-carboxylic acid deaminase.
A method for producing a plant with a reduced level of ethylene which comprises expressing a gene encoding a 1-aminocyclopropane-1-carboxylic acid metabolizing enzyme in said plant at a sufficient level to reduce the steady-state ethylene concentration by at least about 70%, wherein said 1-aminocyclopropane-1-carboxylic acid metabolizing enzyme is 1-aminocyclopropane-1-carboxylic acid deaminase.
Dependencies are inferred from the claim text.