Back to results
US 5,702,925 AGranted
Nucleoside analogue method
AssigneeAmersham International plc
InventorsClifford Smith, Carl Fuller
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeAmersham International plc
InventorsClifford Smith, Carl Fuller
Estimating the term…
A method is provided of making a dideoxynucleoside mono-or tri-phosphate, which is optionally 32-P or 33-P or 35-S radiolabelled which involves reacting the corresponding dideoxynucleoside with an optionally radiolabelled nucleotide phosphate or thiophosphate donor in the presence of a kinase of phosphotransferase enzyme which catalyses the reaction. A method of sequencing nucleic acids by a chain termination of a kinase or phosphotransferase enzyme which catalyses the reaction. A method of sequencing nucleic acids by a chain termination technique involves detecting the products of enzymatic synthesis by means of isotopically labelled chain terminating nucleotide analogues.
A method of making a nucleotide, nucleotide analogue, or nucleotide adduct, having a 5.dbd.-phosphate or a 5'-thiophosphate group said method comprising reacting a starting nucleoside, nucleoside analogue, or nucleoside adduct having a 5'-OH group but no 3'-phosphate group, with a nucleotide phosphate donor or nucleotide thiophosphate donor in the presence of an enzyme which catalyses the reaction.
A kit for sequencing nucleic acids, which comprises each of the four chain terminating nucleotides or nucleotide analogues, wherein the chain terminating nucleotides or nucleotide analogues are labelled with a radioisotope.
A method of sequencing a nucleic acid by a chain-termination technique, which method comprises effecting template-directed enzymatic synthesis using as a chain terminator, a nucleotide or nucleotide analogue labelled with a radioisotope and detecting products of enzymatic synthesis by means of the radioisotope.
A chain terminator selected from the group consisting of ddCTP, ddGTP and ddTTP which is radiolabelled with a member selected from the group consisting of .sup.32 P, .sup.33 P and .sup.35 S.
Dependencies are inferred from the claim text.