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US 5,702,923 AGranted
Method of making a lactococcal bacteriocin
AssigneeQuest International Flavors & Food Ingredients Company, division of Indopco, Inc.
InventorsPeter A. Vandenbergh, Shirley A. Walker, Blair S. Kunka
Life of this patent
Estimating the term…
Abstract
A method of making a bacteriocin produced by Lactococcus lactis NRRL-B-18535 is described. The bacteriocin is useful in foods and other materials and has a wide spectrum of activity against Gram-positive bacteria in a pH range between 2 and 8.
Claims9
A method for producing a bacteriocin in a growth medium which comprises:
- (a) culturing live cells of Lactococcus lactis NRRL-B-18535 in a growth medium for the cells so as to produce the bacteriocin in the growth medium, and wherein the bacteriocin contains a protein having a molecular weight of about 6000 daltons, is inactivated by protease V and not inactivated by alpha-chymotrypsin, trypsin, lipase, pepsin and lysozyme, wherein the bacteriocin inhibits the growth of bacteria selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus carnosus, Pediococcus pentosaceus, Pediococcus acidilactici, Lactococcus cremoris, Lactococcus lactis, Leuconostoc mesenteroides, Lactobacillus bulgaricus, Lactobacillus fermentum, Lactobacillus bifermentans, Lactobacillus plantarum and Listeria monocytogenes and has an pH for inhibition between about pH 2 and 8;
- and
- (b) separating the bacteriocin in the growth medium from the cells.
A method for producing a bacteriocin which comprises:
- (a) culturing live cells of Lactococcus lactis NRRL-B-18535 in a growth medium for the cells to produce the bacteriocin;
- (b) separating the bacteriocin from the cells;
- and
- (c) isolating the bacteriocin from the growth medium, wherein the bacteriocin contains a protein having a molecular weight of about 6000 daltons, is inactivated by protease V and not inactivated by alpha-chymotrypsin, trypsin, lipase, pepsin and lysozyme, wherein the bacteriocin inhibits the growth of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus carnosus, Pediococcus pentosaceus, Pediococcus acidilactici, Lactococcus cremoris, Lactococcus lactis, Leuconostoc mesenteroides, Lactobacillus bulgaricus, Lactobacillus fermentum, Lactobacillus bifermentans, Lactobacillus plantarum and Listeria monocytogenes and has an pH for inhibition between about pH 2 and 8.
1 more element
A method for producing a bacteriocin which comprises:
- (a) culturing live cells of Lactococcus lactis NRRL-B-18535 in a growth medium for the cells, so as to produce the bacteriocin in the growth medium;
- (b) separating bacteriocin from the cells:
- and
- (c) recovering the bacteriocin from the growth medium from the bacterocin by precipitating the bacteriocin in the growth medium and removing the precipitated bacteriocin to provide the bacteriocin, and wherein the bacteriocin contains a protein having a molecular weight of about 6000 daltons, is inactivated by protease V and not inactivated by alpha-chymotrypsin, trypsin, lipase, pepsin and lysozyme, inhibits the growth of bacteria selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus carnosus, Pediococcus pentosaceus, Pediococcus acidilactici, Lactococcus cremoris, Lactococcus lactis, Leuconostoc mesenteroides, Lactobacillus bulgaricus, Lactobacillus fermentum, Lactobacillus bifermentans, Lactobacillus plantarum and Listeria monocytogenes and has an pH for inhibition between about pH 2 and 8.
1 more element
A method for producing a bacteriocin in the growth medium which comprises:
- (a) culturing cells of Lactococcus lactis NRRL-B-18535 in a growth medium for the cells to produce, the bacteriocin in the growth medium;
- (b) filtering the cultured growth medium in a microporous filter means so that the bacteriocin can pass through as a filtrate and which retains components of the growth medium and the cells on the filter means to produce the bacteriocin, and wherein the bacteriocin contains a protein having a molecular weight of about 6000 daltons, is inactivated by protease v and not inactivated by alpha-chymotrypsin, trypsin, lipase, pepsin and lysozyme, inhibits the growth of bacteria selected from the group consisting of Staphylococcus aureus, staphylococcus epidermidis, Staphylococcus carnosus, Pediococcus pentosaceus, Pediococcus acidilactici, Lactococcus cremoris, Lactococcus lactis, Leuconostoc mesenteroides, Lactobacillus bulgaricus, Lactobacillus fermentum, Lactobacillus bifermentans, Lactobacillus plantarum and Listeria monocytogenes and has an pH for inhibition between about pH 2 and 8.
Dependencies are inferred from the claim text.