Method for the extraction of glicentin or glicentin analogous substances
AssigneeNisshin Flour Milling Co., Ltd.
InventorShinjirou Imai
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Abstract
In the method for the extraction of glicentin or a glicentin analogous substance from the cells of a transformed microorganism having incorporated therein an expressible gene encoding glicentin or a glicentin analogous substance, the extraction is carried out using an aqueous solution of an inorganic acid and/or an organic acid. The glicentin is human glicentin or a human glicentin analogous substance.
Claims26
A method for the extraction of glicentin or a glicentin analogous substance from the cells of a transformed microorganism having incorporated therein an expressible gene encoding glicentin or the glicentin analogous substance, which comprises:
- a) extracting said glicentin or glicentin analogous substance from said cells with an aqueous acidic solution;
- and
- b) isolating said glicentin or said glicentin analogous substance.
A method for the extraction of glicentin or a glicentin analogous substance from the cells of a transformed microorganism having incorporated therein an expressible gene encoding the glicentin or the glicentin analogous substance, which comprises:
- a) pretreating the cells of the transformed microorganism with an aqueous acid solution;
- b) separating cell debris and extracting said glicentin or said glicentin analogous substance with the aqueous acid solution;
- and
- c) isolating said glicentin or said glicentin analogous substance.
Dependencies are inferred from the claim text.