Expression of biologically active human C-reactive protein in escherichia coli
AssigneeOrienta Yeast Co., Ltd.
InventorToshio Tanaka
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Abstract
The present invention relates to a plasmid possessing the restriction enzyme map shown in FIG. 1 , and this plasmid is largely characterized in that the kil gene is linked downstream from the human CRP (C-reactive protein) gene. Using this plasmid, it is possible to produce large amounts of human CRP by extracellular secretion.
Claims16
A recombinant plasmid comprising an inducible bacterial promoter which is operably linked to a gene encoding a signal peptide operably linked to the gene encoding human C-reactive protein (CRP).
The recombinant plasmid pTRPCRPS-kil having the pTRPCRPS-kil restriction map set forth in FIG. 1.
An Escherichia coli cell which is transformed with the recombinant plasmid pTRPCRPS-kil.
Dependencies are inferred from the claim text.