DNAS encoding human macrophage migration inhibition factor related peptides

A process for preparing a DNA coding for a compound of formula I or II, a mutant thereof having 1 to 3 codon substitutions or deletions of a fragment thereof comprising at least 15 nucleotides comprising the steps of:

• a) isolating mRNA from human mononuclear leukocytes, selecting the desired mRNA, preparing single-stranded DNA complementary to that mRNA, then double-stranded DNA therefrom (ds cDNA), or
• b) isolating genomic DNA from human cells and selecting the desired DNA using a DNA probe, and
• c) incorporating ds cDNA of step a) or ds cDNA of step b) into an appropriate expression vector,
• d) transforming an appropriate host microorganism with the obtained hybrid vector,
• e) selecting the transformed host which contains DNA coding for a compound of formula I or II, a mutant thereof having 1 to 3 codon substitutions or deletions of a fragment thereof comprising at least 15 nucleotides form hosts containing no coding DNA, and
• f) isolating the desired DNA,
• wherein formula I is ##STR21## wherein Z1 is hydrogen, acyl or the amino acid residue methionine, and wherein formula II is:
• ##STR22## and, wherein Z2 is hydrogen, acyl or an optionally acylated peptide residue of 1 to 5 amino acids.

A DNA encoding a compound of formula I or II, a mutant thereof having 1 to 3 codon deletions or substitutions or a fragment thereof comprising at least 15 nucleotides, wherein said DNA hybridizes with a DNA complementary to formula V or VI,

• wherein formula I is ##STR29## wherein Z1 is hydrogen, acyl or the amino acid residue methionine, wherein formula II is:
• ##STR30## wherein Z2 is hydrogen, acyl or an optionally acylated peptide residue of 1 to 5 amino acids;
• wherein Formula V is:
• ##STR31## wherein Y.sub.1 is a flanking DNA residue of 12 nucleotides or more containing a promoter sequence, and Y.sub.3 is a flanking DNA residue of one or more nucleotides or absent, the complement of said DNA, a mutant of said DNA having 1 to 3 codon deletions or substitutions, a fragment of said DNA comprising at least 15 nucleotides or a genomic DNA wherein the DNA of formula V is interrupted by 1 or 2 introns.
• and wherein Formula VI is:
• ##STR32## wherein Y.sub.1 is a flanking DNA residue of 12 nucleotides or more containing a promoter sequence, Y.sub.2 is ATGACTTGCAAA or absent and Y.sub.3 is a flanking DNA residue of one or more nucleotides or absent, the complement of said DNA, a mutant of said DNA having 1 to 3 codon deletions or substitutions, a fragment of said DNA comprising at least 15 nucleotides or a genomic DNA wherein the DNA of formula VI is interrupted by 1 or 2 introns.