Interruption of binding of MDM2 and p53 protein and therapeutic application thereof
AssigneeUniversity of Dundee
InventorsSteven Michael Picksley, David Philip Lane
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Abstract
A method of identifying a compound which interferes with the binding of MDM2 to human p53 has been determined. This method comprises forming a mixture between MDM2 and a fragment of human p53 consisting of 6 to 28 amino acids comprising TFSDLW (SEQ ID NO:2), adding a test compound to the mixture and determining the quantity of protein bound to the other before and after adding the compound. A compound which decreases the amount of binding of the two proteins to each other is a compound which interferes with the binding of MDM2 to human p53.
Claims5
A method of identifying a compound which interferes with the binding of MDM2 to human p53, said method comprising the steps of:
- forming a mixture by combining a labeled first protein with a second protein, wherein one of the first protein or the second protein is MDM2 and the other is a fragment of human p53 consisting of 6 to 28 amino acids comprising the amino acid sequence TFSDLW (SEQ ID NO:2) or a peptide analogue thereof;
- adding a test compound to the mixture;
- and
- determining the quantity of the first protein which is bound to the second protein before and after said adding step, wherein a decrease in the quantity of the first protein which is bound to the second protein after the adding step indicates that the test compound is a compound which interferes with the binding of MDM2 to human p53.
Dependencies are inferred from the claim text.