Methods for improving the sensitivity of hybridization assays
AssigneeAmoco Corporation
InventorsMark L. Collins, Cecile Blomquist, Massimo Lombardo, John Eldredge
Life of this patent
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Abstract
Methods for improving the sensitivity of hybridization assays which reduce non-specific binding (NSB) and non-specific hybridization (NSH) are disclosed. The methods include a washing method utilizing tetraalkylammonium salts at high temperatures, and release methods in which a probe-target complex is released from a solid support and recaptured. Use of both the washing and release methods results in substantial reduction in NSB and NSH without performing several rounds of release and recapture of the target nucleic acids.
Claims2
A method for increasing the signal to noise ratio in a capture/detection hybridization assay, comprising:
- a) providing a quaternary complex comprising:
- i) a target nucleic acid having a first and a second region;
- ii) a capture probe comprising a first region which is a homopolynucleotide (dA) tail, and a second region which is perfectly complementary to the first region of the target nucleic acid, the second region having a length of from 24 to 40 nucleotides;
- iii) a detection probe which is complementary to the second region of the target nucleic acid;
- and
- iv) a homooligonucleotide of (dT) bound to a solid support, the homooligonucleotide of (dT) having a length of from 10 to 14 nucleotides;
- and
- b) washing the complex of step a) with a solution comprising a tetramethylammonium salt, having a molarity of about 3.0, at a temperature of from 60.degree. C. to 70.degree. C. to elute non-specifically bound detector probe.
A method for increasing the signal to noise ratio in a capture/detection hybridization assay, comprising:
- a) providing a quaternary complex comprising:
- i) a target nucleic acid having a first and a second region;
- ii) a capture probe comprising a first region which is a homopolynucleotide (dT) tail, and a second region which is perfectly complementary to the first region of the target nucleic acid, the second region having a length of from 24 to 40 nucleotides;
- iii) a detection probe which is complementary to the second region of the target nucleic acid;
- and
- iv) a homooligonucleotide of (dA) bound to a solid support, the homooligonucleotide of (dA) having a length of from 10 to 14 nucleotides;
- and
- b) washing the complex of step a) with a solution comprising a tetramethylammonium salt, having a molarity of about 3.0, at a temperature of from 60.degree. C. to 70.degree. C. to elute non-specifically bound detector probe.
Dependencies are inferred from the claim text.