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US 5,702,893 AGranted
Hydrophobic nucleic acid probe
AssigneeChiron Corporation
InventorsMichael S. Urdea, Thomas Horn
Life of this patent
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Abstract
A method for binding a target polynucleotide in a sample is provided. The method uses an assay reagent which is a substrate noncovalently bound to a hydrophobic moiety which is part of a polynucleotide capture probe. This assay reagent is contacted with the sample under conditions which permit hybridization between said capture probe and said target polynucleotide, thereby binding the target polynucleotide.
Claims3
A method for binding a target polynucleotide in a sample to an unsubstituted substrate, said method comprising:
- providing an assay reagent comprising a substrate having bound noncovalently thereto a polynucleotide capture probe having a hydrophobic moiety at an end wherein said capture probe is bound to said unsubstituted substrate through said hydrophobic moiety,
- contacting said assay reagent with said sample under conditions which permit hybridization between said capture probe and said target polynucleotide, thereby binding the target polynucleotide to the unsubstituted substrate.
Dependencies are inferred from the claim text.