HAV probes for use in solution phase sandwich hybridization and assays for detecting the presence of HAV
AssigneeChiron Corporation
InventorsJanice A. Kolberg, Michael S. Urdea
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Abstract
Novel DNA probe sequences for detection of HAV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.
Claims20
A synthetic oligonucleotide useful as an amplifier probe in a sandwich hybridization assay for HAV (Hepatitis A Virus), wherein said oligonucleotide consists of:
- a first segment having a minimum length of 25 nucleotides and a maximum length of 100 nucleotides which segment is at least 90% homologous to a segment of HAV nucleic acid, wherein said first segment comprises a nucleotide sequence selected from the group consisting of SEQ ID NOS:
- 6-27;
- and
- a second segment consisting of a nucleotide sequence which is at least 90% homologous to an oligonucleotide segment of a nucleic acid multimer wherein said second segment is not complementary to HAV nucleic acid;
- and optionally one or more noncomplementary segments each consisting of a nucleotide sequence that is not complementary to HAV nucleic acid.
A synthetic oligonucleotide useful as a capture probe in a sandwich hybridization assay for HAV, wherein the synthetic oligonucleotide consists of:
- a first segment having a minimum length of 25 nucleotides and a maximum length of 100 nucleotides which segment is at least 90% homologous to a segment of HAV nucleic acid, wherein said first segment comprises a nucleotide sequence selected from the group consisting of SEQ ID NOS;
- 28-37;
- and
- a second segment consisting of a nucleotide sequence which is at least 90% homologous to an oligonucleotide bound to a solid phase wherein said second segment is not complementary to HAV nucleic acid;
- and optionally one or more noncomplementary segments each consisting of a nucleotide sequence that is not complementary to HAV nucleic acid.
A set of synthetic oligonucleotides useful as amplifier probes in a sandwich hybridization assay for HAV, comprising at least two different oligonucleotide probes, wherein each oligonucleotide probe consists of:
- a first segment having a minimum length of 25 nucleotides and a maximum length of 100 nucleotides which segment is at least 90% homologous to a segment of HAV nucleic acid, wherein said first segment comprises a nucleotide sequence selected from the group consisting of SEQ ID NOS:
- 6-27;
- and
- a second segment consisting of a nucleotide sequence which is at least 90% homologous to an oligonucleotide segment of a nucleic acid multimer wherein said second segment is not complementary to HAV nucleic acid;
- and optionally one or more noncomplementary segments each consisting of a nucleotide sequence that is not complementary to HAV nucleic acid.
A set of synthetic oligonucleotides useful as capture probes in a sandwich hybridization assay for HAV, comprising at least two different oligonucleotide probes, wherein each oligonucleotide probe consists of:
- a first segment having a minimum length of 25 nucleotides and a maximum length of 100 nucleotides which segment is at least 90% homologous to a segment of HAV nucleic acid, wherein said first segment comprises a nucleotide sequence selected from the group consisting of SEQ ID NOS:
- 28-37;
- and
- a second segment consisting of a nucleotide sequence which is at least 90% homologous to an oligonucleotide bound to a solid phase wherein said second segment is not complementary to HAV nucleic acid;
- and optionally one or more noncomplementary segments each consisting of a nucleotide sequence that is not complementary to HAV nucleic acid.
Dependencies are inferred from the claim text.