Method for the preparation of in vitro-derived human neutrophil precursor cells

A method of preparing an isolated suspension of human neutrophil precursor cells, comprising the steps of:

• a. obtaining CD34+ hematopoietic progenitor cells;
• b. culturing said progenitor cells in the presence of at least one hematopoietic growth factor for between about 7 to about 14 days so as to obtain a suspension of proliferating human neutrophil precursor cells wherein said suspension comprises at least 16% CD15+CD11b- human neutrophil precursor cells and less than about 5% CD34+ colony forming units, and wherein at least about 60% of said precursors are myeloblasts and promyelocytes;
• and
• c. washing said suspension of cells to obtain an isolated cell suspension.