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US 5,700,668 AGranted
Process for the industrial preparation of phosphatidylserine
AssigneeItalfarmaco Sud S.p.A.
InventorsLorenzo De Ferra, Pietro Massardo, Oreste Piccolo, Stefano Servi
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Abstract
The invention relates to a process for the preparation of phosphatidylserines by reacting racemic or enantiomerically pure serine, preferably (L)-serine, with natural phosphatides, such as soybean or egg lecithin, or with synthetic phosphatides, in the presence of a phospholipase D, having transphosphatidylating activity, in an aqueous/organic diphasic system.
Claims16
A process for the preparation of phosphatidylserines compounds of formula (I) ##STR2## wherein:
- R.sup.1 and R.sup.2, which are the same or different, are a C.sub.10 -C.sub.30 acyl;
- X.dbd.OH or OM, wherein M is an alkali, alkaline-earth metal, ammonium or alkylammonium;
- which process comprises reacting phosphatides of general formula (II) ##STR3## wherein R.sup.1, R.sup.2 and X have the above defined meanings and R.sup.3 .dbd.CH.sub.2 --CH.sub.2 NH.sub.2 or CH.sub.2 --CH.sub.2 N.sup.+ (CH.sub.3).sub.3, with racemic or enantiomerically pure serine, in a water/organic solvent diphasic system, in the presence of crude phospholipase D from centrifuged fermentation broths of microorganisms strains producing extracellular PLD having a high transphosphatidylation activity.
Dependencies are inferred from the claim text.