Method of detection of urinary tumor associated antigen
OwnerDonald L. Morton, Rishab K. Gupta +1inventors
InventorsDonald L. Morton, Rishab K. Gupta, David M. Euhus
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Abstract
The present invention describes Urinary Tumor Associated Antigen (UTAA), its isolation and use in diagnostic assays. In particular, UTAA has been identified in samples from cancer patients, in some cases as part of an immune complex of UTAA and UTAA-specific immunoglobulin. Isolated UTAA also may be formulated as a pharmaceutical for production of antibodies or as a vaccine.
Claims24
A method of detecting a cancer in a subject having a naturally occurring immune complex of Urinary Tumor Associated Antigen (UTAA) and a first anti-UTAA antibody comprising
- (i) contacting a sample from said subject with a second anti-UTAA antibody;
- and
- (ii) detecting said complexes bound to said second anti-UTAA antibody with an antibody reactive with said first anti-UTAA antibody,
- wherein said first and said second anti-UTAA antibodies recognized different epitopes on UTAA.
A method for monitoring a malignancy in a subject having a naturally occurring immune complex of Urinary Tumor Associated Antigen (UTAA) and a first anti-UTAA antibody comprising
- (i) contacting a sample from said subject with a second anti-UTAA antibody;
- (ii) detecting said complexes bound to said second anti-UTAA antibody with an antibody reactive with said first anti-UTAA antibody, wherein said first and said second anti-UTAA antibodies recognize different epitopes on UTAA;
- (iii) determining the amount of UTAA per a given unit of body fluid;
- and
- (iv) comparing the amount with an amount previously determined for an equivalent sample,
- wherein the variation in UTAA amount indicates a variation in the state of the malignancy.
A method of detecting Urinary Tumor Associated Antigen in a sample comprising:
- (1) contacting the sample with a first reagent which binds to a first epitope on Urinary Tumor Associated Antigen;
- (2) contacting the sample with a second reagent which binds to a second epitope on Urinary Tumor Associated Antigen;
- and
- (3) detecting the presence of the first or second bound reagent, thereby detecting the presence of Urinary Tumor Associated Antigen.
A method of detecting Urinary Tumor Associated Antigen (UTAA) in a sample comprising:
- (1) contacting the sample with a first reagent which binds to an epitope on UTAA selected from the group consisting of the epitope on the 45, 65, 90-100 and 120 kD subunit as identified after reduction by .beta.-mercaptoethanol and separation by SDS-polyacrylamide gel electrophoresis;
- (2) contacting the sample with a second reagent which binds to a second epitope on UTAA selected from the group consisting of the epitope on the 45, 65, 90-100 and 120 kD subunit as identified after reduction by .beta.-mercaptoethanol and separation by SDS-polyacrylamide gel electrophoresis;
- and
- (3) detecting said second reagent bound to said sample.
A method for detecting a naturally-occurring immune complex of a urinary tumor associated antigen (UTAA) and a first anti-UTAA antibody in a sample comprising the steps of:
- (i) contacting said sample with a second anti-UTAA antibody;
- and
- (ii) detecting said complexes bound to said second anti-UTAA antibody with an antibody reactive with said first anti-UTAA antibody, wherein said first and said second anti-UTAA antibodies recognize different epitopes on UTAA.
Dependencies are inferred from the claim text.