Carbocation containing cyanine-type dye
AssigneeCanon Kabushiki Kaisha
InventorsTakeshi Miyazaki, Kazumi Tanaka, Tsuyoshi Santo, Toshikazu Ohnishi, Tetsuro Fukui, Tadashi Okamoto
Life of this patent
Estimating the term…
Abstract
To provide a reagent with excellent stability under storage, which can detect a subject compound to be measured with higher specificity and sensitibity. Complexes of a compound represented by the general formula (IV): ##STR1##
Claims20
A labeled complex for detecting a subject compound to be analyzed by means of optical means using near-infrared radiation which complex comprises a substance from a living organism and a labeling agent fixed onto the substance, the substance capable of specifically binding to the subject compound, wherein the labeling agent comprises a compound represented by the general formula (IV):
- ##STR99## wherein A, B, D and E are independently selected from the group consisting of hydrogen atom, a substituted or an unsubstituted alkyl group having two or more carbon atoms, alkenyl group, aralkyl group, aryl group, styryl group and heterocyclic group, and at least one of A and B is a substituted or unsubstituted aryl group, and at least one of D and E is a substituted or unsubstituted aryl group;
- r.sub.1 ' and r.sub.2 ' are individually selected from the group consisting of hydrogen atom, a substituted or an unsubstituted alkyl group, cyclic alkyl group, alkenyl group, aralkyl group and aryl group;
- k is 0 or 1;
- is 0, 1 or 2;
- and X.sub.2.sup..crclbar. represents an anion.
A method of detecting a subject compound to be analyzed in a sample comprising the steps of:
- providing a labeled complex comprising a substance from a living organisms and a labeling agent fixed onto the substance, the substance being capable of specifically binding to the subject compound;
- binding the labeled complex to the subject compound;
- and
- detecting the labeled complex to which the subject compound is bonded by means of optical means, wherein the labeling agent comprises a compound represented by the general formula (IV):
- ##STR101## wherein A, B, D and E are independently selected from the group consisting of hydrogen atom, a substituted or an unsubstituted alkyl group having two or more carbon atoms, alkenyl group, aralkyl group, aryl group, styryl group and heterocyclic group, and at least one of A and B is a substituted or unsubstituted aryl group, and at least one of D and E is a substituted or unsubstituted aryl group;
- r.sub.1 ' and r.sub.2 ' are individually selected from the group consisting of hydrogen atom, a substituted or an unsubstituted alkyl group, cyclic alkyl group, alkenyl group, aralkyl group and aryl group;
- k is 0 or 1;
- is 0, 1 or 2;
- and X.sub.2.sup..crclbar. represents an anion.
Dependencies are inferred from the claim text.