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AssigneePromega Corporation
InventorsEdward E. Pahuski, Randall L. Dimond, John H. Priest, Lisa Zandt, Kathleen K. Stebnitz, Leopoldo G. Mendoza
Estimating the term…
Processes are provided for removal of cells as cell pellets from liquid milk samples, or from cultures or extracts of other food materials or other materials of biological origin. The concentrated cells in the pellet can be analyzed by various techniques to determine the relative cell count, such as by lysing of the cells followed by measurement of ATP. Nucleic amplification, as by the polymerase chain reaction method, can be carried out using the cellular pellet directly, without need for isolation of nucleic acid from the cells. After the amplification, an assay can be carried out for amplified nucleic acid segment indicative of the presence of cells of interest in the sample. The invention thus provides methods for obtaining cellular components from samples of milk, and cultures or extracts of other materials, including food materials, and for determining relative contamination of milk and such other materials by microorganisms. The invention also provides kits for carrying out its various methods.
A method of separating and concentrating cells from a liquid milk sample comprising the steps of:
A microbial test kit for use in testing milk samples, comprising:
A test kit for use in testing milk samples for somatic cells, comprising:
A method of treating a liquid milk sample whereby cells in the sample are separated from other milk components and concentrated, comprising the steps of:
A clearing solution for use in testing milk samples, comprising a chelating agent, a microparticulate carrier wherein the microparticulate carrier sediments slightly slower than microbial cells, and wherein the microparticulate carrier serves as a visual indicator facilitating the removal of supernatant without disturbing the cell pellet in the milk sample and a somatic cell lysing agent.
A clearing solution comprising an aliquot of a culture or extract of a material of biological origin, said solution combined with a microparticulate carrier wherein the microparticulate carrier sediments slightly slower than microbial cells, and wherein the microparticulate carrier serves as a visual indicator facilitating the removal of supernatant without disturbing the cell pellet of the culture or the extract, wherein said microparticulate carrier is in suspension in the solution.
A method of separating and concentrating cells from an aliquot of a culture or extract of a material of biological origin comprising the steps of:
Dependencies are inferred from the claim text.