Recombinant adenovirus with removed EZA gene and method of preparation
AssigneeSumitomo Pharmaceuticals Company, Limited
InventorsIzumu Saito, Yumi Kanegae, Michio Nakai
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Abstract
A recombinant DNA virus for transfecting an animal cell and bearing a foreign gene and a promoter capable of regulating expression of the foreign gene is completely deleted of the function of E2A gene. The recombinant DNA virus can thus stably transduce the foreign gene into various animal cells, which leads to continuous expression of the foreign gene in the animal cells. The continuous expression of the foreign gene can provide an effective treatment of hereditary disease.
Claims31
A recombinant adenovirus comprising an adenovirus genome having a foreign gene and a promoter for expressing said foreign gene, wherein the function of an E2A gene is completely deleted by removing a part or all of said E2A gene.
The recombinant adenovirus of claim 1 or 8 wherein said foreign gene and said promoter for expressing said foreign gene are inserted in a deletion.
A recombinant adenovirus comprising an adenovirus genome having two recombinase-recognition sequences in the same orientation located on both sides of an E2A gene.
A method for constructing a recombinant adenovirus without a functional E2A gene, which comprises the steps of:
- transducing an animal cell line with an adenovirus vector (a) having inserted therein a promoter, a recombinase gene and a poly(A) sequence, and a recombinant adenovirus (b) comprising an adenovirus genome having two recombinase-recognition sequences located in the same orientation on both sides of an E2A gene region and
- excising the E2A gene region located between said two recombinase-recognition sequences.
A recombinant adenovirus comprising an adenovirus genome having a foreign gene inserted between the termination codons of an E2A gene and an L3 gene.
Dependencies are inferred from the claim text.