Chemotactic peptide pharmaceutical applications
AssigneeRhomed Incorporated
InventorsPaul O. Zamora, Richard J. Freer
Life of this patent
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Abstract
High affinity chemotactic peptides containing a biological-function domain which includes at least two linked N-formyl-Met-Leu-Phe sequences and a medically useful metal ion-binding domain are labeled with medically useful metal ions for use in a variety of diseases and pathologic conditions, and particularly for diagnosis and treatment of infections, inflammations and other diseases and conditions.
Claims13
A high affinity chemotactic peptide-based pharmaceutical composition suitable for administration to a patient comprising:
- a biological-function domain including at least two linked N-formyl-Met-Leu-Phe sequences wherein the sequences are linked by means of peptide side chains;
- and
- a medically useful metal ion-binding domain comprising a sequence of amino acids containing sulfur or nitrogen atoms which are available for metal ion binding.
A high-affinity chemotactic peptide-based pharmaceutical composition suitable for administration to a patient comprising:
- a biological-function domain;
- and
- a medically useful metal ion-binding domain;
- wherein said composition is selected from the group consisting of
- (R.sub.1)--Y.sub.1 !.sub.n --(R.sub.2),
- (R.sub.1)--Y.sub.1 --(R.sub.2)--Y.sub.1 !.sub.n --(R.sub.3)
- and (R.sub.1)--Y.sub.1 --(R.sub.2)--Y.sub.2 !.sub.n --(R.sub.3)
- wherein the medically useful metal ion-binding domain is, respectively, Y.sub.1 !.sub.n, Y.sub.1 --(R.sub.2)--Y.sub.1 !.sub.n and Y.sub.1 --(R.sub.2)--Y.sub.2 !.sub.n in which n is a number between 1 and about 6 and Y.sub.1 and Y.sub.2 are amino acids comprising a sulfur, nitrogen or oxygen which is available for binding to a metal ion;
- wherein the biological-function domain comprises at least one, or a portion of one, of the group consisting of amino acid sequences R.sub.1, R.sub.2 and R.sub.3, which comprises at least two linked N-formyl-Met-Leu-Phe sequences;
- wherein those portions of R.sub.1, R.sub.2 and R.sub.3 not comprising the biological-function domain each comprise an amino acid sequence containing from 0 to about 20 amino acids;
- wherein said medically useful metal ion-binding domain comprises at least one amino acid sequence consisting of at least one amino acid selected from the group consisting of cysteine, cystine, histidine, penicillamine, deacylated methionine, lysine, arginine, aspartic acid, glutamic acid, tyrosine, Cys!.sub.n, Cys--(R.sub.2)--Cys!.sub.n, Cys--(R.sub.2)--Pen!.sub.n, His--(R.sub.2)--Cys!.sub.n, His--(R.sub.2)--Pen!.sub.n, His!.sub.n and His--(R.sub.2)--His!.sub.n,
- wherein n is a number between 1 and about 6;
- and R.sub.2 is independently an amino acid sequence containing from 1 to about 20 amino acids.
Dependencies are inferred from the claim text.