Crosslinked polyacrylamide gels with high monomer: crosslinker ratios
AssigneeAmresco
InventorsTimothy J. Highman, Michael E. Smerdel, Stephen M. Behm
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Abstract
Improved polyacrylamide-type gels utilizing diacrylyl tertiary amide crosslinkers are disclosed having relatively high monomer:crosslinker ratios in comparison to conventional polyacrylamide gels. The resulting gels have pore structures chemically and physically different from conventional polyacrylamide gels and, when prepared in the optimal monomer:crosslinker ratio, provide superior resolution and structural characteristics over the conventional polyacrylamide gels.
Claims14
A composition for separating and detecting molecules comprising a gel which comprises an acrylamide monomer crosslinked with an amine acryloyl derivative of an amino compound having at least one secondary amine group, said derivative also having at least one tertiary amide group, wherein the ratio of acrylamide monomer:acryloyl derivative ranges from 40:1 to about 150:1, and wherein the concentration of the gel in the composition is between 3.0% and 25.0% by weight of the composition.
A method for detecting molecules comprising
- placing a sample containing molecules onto a composition comprising a gel which comprises an acrylamide monomer crosslinked with an amine acryloyl derivative of an amino compound having at least one secondary amine group, said derivative also having at least one tertiary amide group, wherein the ratio of acrylamide monomer:acryloyl derivative ranges from 40:1 to about 150:1, and wherein the concentration of the gel in the composition is between 3.0% and 25.0% by weight of the composition,
- contacting the composition with a solvent for a sufficient length of time to cause the molecules to migrate differentially through the composition a predetermined distance, and
- contacting the composition with a silver solution to develop the composition and detect the relative positions of the molecules in the sample.
Dependencies are inferred from the claim text.