DNA encoding the pseudomonas fluorescens sensor/kinase element, lemA, and its use in activating gene expression
AssigneeNovartis Finance Corporation
InventorsStephen T. Lam, Thomas D. Gaffney
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Abstract
Gene activating sequences which activate the expression of other bacterial genes, which are latent or expressed at low levels, are provided. The gene activating sequences confer the ability to produce several metabolites and may be transferred to bacterial strains. The transformed biocontrol agents are active to inhibit the growth of the fungal pathogens.
Claims12
An isolated gene activating element comprising a nucleotide sequence capable of inducing the expression of at least one gene in a Pseudomonad selected for transformation, wherein said gene is latent or expressed at low levels in said Pseudomonad, and wherein said nucleotide sequence is a Pseudomonas fluorescens lemA sequence comprised by the DNA fragment deposited as pCIB168.
An isolated gene activating element comprising a nucleotide sequence capable of inducing the expression of at least one gene in a Pseudomonad selected for transformation, wherein said gene is latent or expressed at low levels in said Pseudomonad, and wherein said nucleotide sequence encodes the polypeptide encoded by a Pseudomonas fluorescens lemA sequence comprised by the DNA fragment deposited as pCIB168.
Dependencies are inferred from the claim text.