Regulatory gene for the expression of nitrile hydratase gene
AssigneesSakayu Shimizu, Nitto Chemical Industry Co., Ltd.
InventorsSakayu Shimizu, Michihiko Kobayashi
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Abstract
The invention relates to a regulatory gene coding for a polypeptide having the ability to activate a promoter for a nitrile hydratase gene, a recombinant DNA containing said regulatory gene, and a transformant transformed with said recombinant DNA. The introduction of the regulatory gene of the invention along with a nitrile hydratase gene and its promoter region permits bacteria of the genus Rhodococcus to produce a higher level of nitrile hydratase. Other extraneous gene can also be introduced into a region downstream of the promoter to produce other proteins in high yield.
Claims4
An isolated regulatory gene coding for a polypeptide having the ability to activate a promoter for a nitrile hydratase gene and having the amino acid sequence defined in the Sequence Listing by SEQ ID No:
- 1.
Dependencies are inferred from the claim text.