A test structure enabling calibration of imaging instruments for measurement of overlay as experienced during fabrication of products formed in multiple-step processes, measurements of overlay as made by such imaging instruments being susceptible to one or both of tool-induced shift (TIS) and wafer-induced shift (WIS), said structure comprising a first component formed in a first step and at least a second component formed in a second step, said first and second components together defining a number of artifacts on said substrate, an electrical characteristic of each of said artifacts varying in accordance with the relative alignment of the first and second components thereof, such that the relative alignment of said first and second components comprised by each of said artifacts are measurable using said imaging instrument to be calibrated and using electrical measurement techniques not susceptible to TIS and WIS.