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US 5,574,206 AGranted
Transgenic mouse carrying a non-infectious HIV genome
AssigneeL'Institut de Recherches Cliniques de Montreal
InventorPaul Jolicoeur
Life of this patent
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Abstract
Disclosed herein is a transgenic mouse carrying a transgene which expresses non-infectious HIV ribonucleic acid, and the complementary proteins thereof. The transgenic mouse is useful as a source for obtaining the complementary proteins, and as an animal model to study HIV host cell interactions and to evaluate anti-HIV drugs.
Claims3
A transgenic mouse in which the germ cells and somatic cells carry at least one copy of a single transgene that comprises:
- (i) a HIV DNA genome from which is deleted the 5'-LTR, a portion of the 5' leader sequence, and a portion of the 3'-LTR, whereby said HIV DNA and its RNA transcript are rendered non-infectious,
- (ii) the MMTV LTR promoter operatively linked to the 1' end of said DNA, and
- (iii) the SV40 poly(A) addition signal operatively linked to the 3' end of said DNA, wherein said transgene is introduced into said mouse or an ancestor thereof as a single transgene, such that said mouse has the entire coding sequence of said HIV DNA genome, but produces non-infectious HIV RNA, said transgenic mouse further expressing (a) detectable levels of RNA transcripts encoding HIV proteins env, gag and pol in a promoter-specific manner, and (b) detecting levels of HIV structural proteins env and gag.
A transgenic mouse in which the germ cells and somatic cells carry at least one copy of a single transgene that comprises:
- (i) a HIV DNA genome from which is deleted the 5'-LTR, a portion of the 5' leader sequence, and a portion of the 3'-LTR, whereby said HIV DNA and its RNA transcript are rendered non-infectious,
- (ii) the NF-L promoter operatively linked to the 5' end of said DNA, and
- (iii) the SV40 poly(A) addition signal operatively inked to the 3' end of said DNA, wherein said transgene is introduced into said mouse or an ancestor thereof as a single transgene, such that said mouse has the entire coding sequence of said HIV DNA genome, but produces non-infectious HIV RNA, said transgenic mouse further expressing (a) detectable levels of RNA transcripts encoding HIV proteins env, gag and pol in a promoter-specific manner, and (b) detectable levels of HIV structural protein gag in spinal cord and brain tissues.
Dependencies are inferred from the claim text.