Method for isolating biomolecules from a biological sample with linear polymers
AssigneeMiddlesex Sciences, Inc.
InventorJames E. Woiszwillo
Life of this patent
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Abstract
A method and composition for isolating biomolecules from a biological sample wherein the sample is mixed with a soluble, linear polymer, such as polyvinylpyrrolidone, to form a precipitate. The biomolecule of interest is found in the precipitate or is isolated from the supernatant.
Claims25
A method of isolating at least one biomolecule from a biological sample comprising the steps of:
- a) adding a sufficient amount of a solution of at least two soluble, linear polymers to a biological sample solution to form a precipitate, wherein a first polymer is polyvinylpyrrolidone and a second polymer is polyethylene glycol, and adjusting the solution to a predetermined pH;
- and
- b) separating the precipitate from the supernatant;
- thereby isolating a biomolecule from the biological sample in either the supernatant or precipitate.
An assay for determining the albumin to globulin ratio in plasma comprising the steps of:
- a) adding a sufficient amount of a polymer solution of polyvinylpyrrolidone and polyethylene glycol to the plasma to precipitate the globulin, leaving the albumin in the supernatant, wherein the pH of the plasma is adjusted to an approximately neutral pH;
- b) separating the precipitate from the supernatant;
- c) measuring the concentrations of globulin and albumin;
- and
- d) calculating the ratio of albumin to globulin.
A method for determining the disposition of a drug in serum proteins comprising the steps of:
- a) adding a sufficient amount of an aqueous solution of polyvinylpyrrolidone and polyethylene glycol to serum to precipitate the globulin, wherein the pH of the serum is adjusted to a predetermined value;
- b) separating the precipitate from the supernatant, thereby isolating globulin-bound drug and albumin-bound drug in either the precipitate or supernatant;
- and
- c) analyzing the precipitate and supernatant to detect the drug.
A method of isolating at least one protein from a biological sample solution comprising the steps of:
- a) combining polyvinylpyrrolidone with polyethylene glycol to form a polymer mixture;
- b) adjusting the pH of the polyvinylpyrrolidone and polyethylene glycol mixture to a predetermined value with an acid or base;
- c) adding a sufficient amount of the pH-adjusted polyvinylpyrrolidone and polyethylene glycol mixture to the sample solution to form a precipitate;
- and
- d) separating the precipitate from the supernatant, thereby isolating a protein from the biological sample in either the supernatant or precipitate.
Dependencies are inferred from the claim text.