Method for the colorimetric determination of an analyte by means of benzyl alcohol dehydrogenase and a chromogenic redox indicator

A nitrosoaniline compound of the formula I, ##STR54## wherein R.sup.1 is selected from the group consisting of hydrogen, halogen, hydroxy, alkoxy, aryloxy, alkylthio or arylthio, an alkyl or alkylene which is unsubstituted or substituted by carboxy, PO.sub.3 H.sub.2, dialkylphosphinyl or SO.sub.3 H, and an amino which is unsubstituted or substituted once or twice by an alkyl which in turn can be unsubstituted or substituted by hydroxy, PO.sub.3 H.sub.2, SO.sub.3 H or carboxy, and

• R is a residue which produces a color by electron conjugation with a quinone diimiine system.

A reagent for the colorimetric determination of NAD(P)H comprising benzyl alcohol dehydrogenase and a nitrosoaniline compound of formula I:

• ##STR62## wherein R.sup.1 is selected from the group consisting of hydrogen, halogen, hydroxy, alkoxy, aryloxy, alkylthio or arylthio, alkyl or alkylene unsubstituted or substituted by carboxy, PO.sub.3 H.sub.2, dialkylphosphinyl or SO.sub.3 H, and an amino unsubstituted or substituted once or twice by alkyl which in turn can be unsubstituted or substituted by hydroxy, PO.sub.3 H.sub.2, SO.sub.3 H or carboxy, and
• R is a residue which forms a color by electron conjugation with a quinone diimiine system.

A reagent for the colorimetric determination of benzyl alcohol dehydrogenase comprising NAD(P)H and a direct chromogenic electron acceptor, wherein the electron acceptor is a nitrosoaniline compound of formula I:

• ##STR63## wherein R.sup.1 is selected from the group consisting of hydrogen, halogen, hydroxy, alkoxy, aryloxy, alkylthio or arylthio, alkyl or alkylene unsubstituted or substituted by carboxy, PO.sub.3 H.sub.2, dialkylphosphinyl or SO.sub.3 H, and an amino unsubstituted or substituted once or twice by alkyl which in turn can be unsubstituted or substituted by hydroxy, PO.sub.3 H.sub.2, SO.sub.3 H or carboxy, and
• R is a residue which forms a color by electron conjugation with a quinone diimiine system.

A reagent for the colorimetric determination of a NAD(p).sup.+ -dependent dehydrogenase comprising a substrate of the dehydrogenase to be determined, NAD(P).sup.+, an NAD(P)H-converting dehydrogenase and a direct chromogenic electron acceptor,

• wherein the NAD(P)H-converting dehydrogenase is benzyl alcohol dehydrogenase and wherein the electron acceptor is a nitrosoaniline compound of formula I:
• ##STR64## wherein R.sup.1 is selected from the group consisting of hydrogen, halogen, hydroxy, alkoxy, aryloxy, alkylthio or arylthio, alkyl or alkylene unsubstituted or substituted by carboxy, PO.sub.3 H.sub.2, dialkylphosphinyl or SO.sub.3 H, and an amino unsubstituted or substituted once or twice by alkyl which in turn can be unsubstituted or substituted by hydroxy, PO.sub.3 H.sub.2, SO.sub.3 H or carboxy, and
• R is a residue which forms a color by electron conjugation with a quinone diimiine system.

A reagent for the colorimetric determination of an analyte comprising an analyte-specific NAD(P)-dependent dehydrogenase, NAD(P), an NAD(P)H-oxidizing enzyme and a direct chromogenic electron acceptor, wherein said NAD(P)H-oxidizing enzyme is benzyl alcohol dehydrogenase and said direct chromogenic electron acceptor is a nitrosoaniline compound of formula I:

• ##STR65## wherein R.sup.1 is selected from the group consisting of hydrogen, halogen, hydroxy, alkoxy, aryloxy, alkylthio or arylthio, alkyl or alkylene unsubstituted or substituted by carboxy, PO.sub.3 H.sub.2, dialkylphosphinyl or SO.sub.3 H, and an amino unsubstituted or substituted once or twice by alkyl which in turn can be unsubstituted or substituted by hydroxy, PO.sub.3 H.sub.2, SO.sub.3 H or carboxy, and
• R is a residue which forms a color by electron conjugation with a quinone diimiine system.