Array of oligonucleotides on a solid substrate
AssigneeAffymax Technologies N.V.
InventorsStephen P. A. Fodor, Michael C. Pirrung, J. Leighton Read, Lubert Stryer
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Abstract
A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, is provided to the substrate to bind at the selected areas. The process is repeated using a variety of monomers such as amino acids until sequences of a desired length are obtained. Detection methods and apparatus are also disclosed.
Claims10
A substrate with a surface comprising 10.sup.3 or more groups of oligonucleotides with different, known sequences covalently attached to the surface in discrete known regions, said 10.sup.3 or more groups of oligonucleotides occupying a total area of less than 1 cm.sup.2 on said substrate, said groups of oligonucleotides having different nucleotide sequences.
An array of more than 1,000 different groups of oligonucleotide molecules with known sequences covalently coupled to a surface of a substrate, said groups of oligonucleotide molecules each in discrete known regions and differing from other groups of oligonucleotide molecules in monomer sequence, each of said discrete known regions being an area of less than about 0.01 cm.sup.2 and each discrete known region comprising oligonucleotides of known sequence, said different groups occupying a total area of less than 1 cm.sup.2.
Dependencies are inferred from the claim text.