Cartridge, apparatus, and method for preparing purified nucleic acids from a cell sample
AssigneeBertin & Cie
InventorJean Boquet
Life of this patent
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AssigneeBertin & Cie
InventorJean Boquet
Estimating the term…
A cartridge for preparing nucleic acids such as DNA from cells comprises two end rings (14) enabling it to be mounted in sealed manner in a tube (10), two dialysis membranes (16) delimiting a dialysis enclosure (18) between them, a filter (20) for retaining cell nuclei dividing said enclosure (18) into two separate compartments, and means (24) for feeding substances into one of the compartments, means (26) for extracting substances from the other compartments, and means (28, 30) for feeding a dialysis liquid into the tube outside the cartridge, or for causing it to flow therethrough. The invention also provides apparatus, and a method using said cartridge.
A cartridge for preparing purified nucleic acids from cells, the cartridge comprising a dialysis enclosure, a filter provided in the dialysis enclosure for separating it into two separate compartments, the filter being in a material adapted for retaining cell nuclei, and inlet and outlet means for admitting and extracting substances into and out from the dialysis enclosure, said enclosure comprising a elongate plane support having a central slot and two outer faces, two dialysis membranes fixed on the outer faces of the support for defining the dialysis enclosure between them, and two end rings mounted on longitudinally opposite ends of the support and each comprising a through duct that opens out into the dialysis enclosure, the through duct of one of the end rings opening out on a side of the filter and the through duct of the other end ring opening out on an opposite side of the filter.
A method of preparing purified nucleic acids from cells, by lysis of the cell nuclei, by degrading proteins, and by purifying the nucleic acids by dialysis, comprising:
Dependencies are inferred from the claim text.