Isoamylase and process for producing the same
AssigneeAmano Pharmaceutical Co., Ltd.
InventorsYuzo Yamada, Toshihiro Sato, Takaichi Ohya
Life of this patent
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Abstract
An isoamylase, useful in the field of starch saccharification, having a molecular weight of about 105,000, an isoelectric point of 6.4, an optimum pH of 3.0 to 5.0, an optimum temperature of about 50.degree. C. and exhibiting temperature stability at 45.degree. C..times.10 minutes and pH stability at pH 3.5 to 6.0, and a process for producing the same comprising cultivating an isoamylase-producing strain belonging to the genus Xanthomonas and recovering the enzyme produced.
Claims7
Substantially pure isoamylase produced by cultivating an isoamylase-producing Xanthomonas maltophilia strain, said substantially pure isoamylase having the following enzymological and chemical properties:
- 1) Substrate specificity:
- actively acts on glycogen, also acts on amylopectin, hardly acts on pullulan,
- 2) Optimum pH:
- 3.0 to 5.0,
- 3) pH Stability:
- pH 3.5 to 6.0,
- 4) Optimum Temp.:
- around 50.degree. C.,
- 5) Temperature Stability:
- stable at 45.degree. C. for 10 minutes,
- 6) Isoelectric point:
- 6.4, and
- 7) Molecular weight:
- about 105,000, as measured by gel-filtration on SUPEROSE 12.
A process for producing an isoamylase having the following enzymological and chemical properties:
- 1) Substrate specificity:
- actively acts on glycogen, also acts on amylopectin, hardly acts on pullulan,
- 2) Optimum pH:
- 3.0 to 5.0,
- 3) pH Stability:
- pH 3.5 to 6.0,
- 4) Optimum Temp.:
- around 50.degree. C.,
- 5) Temperature Stability:
- stable at 45.degree. C. for 10 minutes,
- 6) Isoelectric point:
- 6.4, and
- 7) Molecular weight:
- about 105,000, as measured by gel-filtration on SUPEROSE 12,
- comprising cultivating an isoamylase-producing strain belonging to the genus Xanthomonas and recovering the isoamylase produced.
Dependencies are inferred from the claim text.