Myod regulatory region

A method of positively selecting for muscle cells, comprising:

• constructing a DNA or RNA expression vector comprising a myoD proximal regulatory region and a myoD distal regulatory region both operably linked to a marker gene whereby transcription of the marker gene is controlled by the myoD proximal and distal regulatory regions,
• introducing the expression vector into the genomes of a population of cells, and
• positively selecting for cells that express the marker,
• wherein the myoD proximal regulatory region is DNA or RNA capable of hybridizing under stringent conditions to the nucleotide sequence shown from positions 590 to 883 in SEQ ID NO:
• 1 and wherein the myoD distal regulatory region is DNA or RNA capable of hybridizing under stringent conditions to the nucleotide sequence shown from positions 1 to 1200 in SEQ ID NO:
• 2.

A method of negatively selecting for a muscle cell, comprising:

• constructing a DNA or RNA expression vector comprising a myoD proximal regulatory region and a myoD distal regulatory region both operably linked to a gene encoding a toxin whereby transcription of the gene is controlled by the myoD proximal and distal regulatory regions, and
• introducing the vector into the genome of the cell,
• wherein the myoD proximal regulatory region is DNA or RNA capable of hybridizing under stringent conditions to the nucleotide sequence shown from positions 590 to 883 in SEQ ID NO:
• 1 and wherein the myoD distal regulatory region is DNA or RNA capable of hybridizing under stringent conditions to the nucleotide sequence shown from positions 1 to 1200 in SEQ ID NO:
• 2.

A method of negatively selecting for muscle cells, comprising:

• introducing a DNA or RNA expression vector into the genomes of a population of cells, the expression vector comprising a myoD proximal regulatory region and a myoD distal regulatory region both operably linked to a gene encoding a protein for converting a prodrug to a toxic drug whereby transcription of the gene is controlled by the myoD proximal and distal regulatory regions, and
• contacting the cells with the prodrug,
• wherein the myoD proximal regulatory region is DNA or RNA capable of hybridizing under stringent conditions to the nucleotide sequence shown from positions 590 to 883 in SEQ ID NO:
• 1 and wherein the myoD distal regulatory region is DNA or RNA capable of hybridizing under stringent conditions to the nucleotide sequence shown from positions 1 to 1200 in SEQ ID NO:
• 2.