Aldolase enzyme process for making 5-azido-5-deoxy-hexulose-1-phosphate
AssigneeThe Scripps Research Institute
InventorsChi-Huey Wong, Kun-Chin Liu
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Abstract
2-Methyl-5-hydroxymethyl-and 2,5-dimethyl-3,4-dihydroxypyrrolidines, their processes of preparation and use are disclosed. 5-Azido-5-deoxy-hexulose-1-phosphate compounds and processes of making the same are also disclosed.
Claims6
A process of making a 5-azido-5-deoxyhexulose-1-phosphate comprising mixing a 2-azido-substituted-propionaldehyde and dihydroxyacetone phosphate in the presence of a catalytic amount of an aldolase to form a reaction mixture and maintaining the reaction mixture under biological reaction conditions for a time period sufficient to form said 5-azido-5-deoxy-hexulose-1-phosphate.
A process of making a 5-azido-5-deoxy-hexulose-1-phosphate comprising mixing a 2-azido-substituted-propionaldehyde and dihydroxyacetone phosphate in the presence of a catalytic amount of an aldolase that is selected from the group consisting of fructose-1,6-diphosphate aldolase, rhamnulose-1-phosphate aldolase, fuculose-1-phosphate aldolase and tagatose-1,6-diphosphate aldolase to form a reaction mixture and maintaining the reaction mixture under biological reaction conditions for a time period sufficient to form said 5-azido-5-deoxy-hexulose-1-phosphate.
Dependencies are inferred from the claim text.