US 5,350,694 AGranted

Composition method and device for measuring the divalent cation concentration or specific gravity of a test sample

AssigneeMiles Inc.

InventorChris T. Zimmerle

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Abstract

A composition, method and test device for determining the divalent cation concentration or estimating the specific gravity of a test sample are disclosed. The method utilizes a test device comprising a test pad, wherein the test pad includes a carrier matrix incorporating a reagent composition capable of interacting with divalent cations present in the test sample to produce a detectable and measurable response that correlates to the divalent cation concentration or to the specific gravity of the test sample. The reagent composition, comprising a metal-sensitive triphenylmethane dye; a buffer; an optional chelating agent; and suitable carrier, is incorporated into a carrier matrix to provide a test pad of a device useful in a divalent cation assay or a specific gravity assay of a test sample.

Claims19

Dependent claims

A composition capable of exhibiting a detectable and measurable color transition only in response to a total divalent cation concentration of an aqueous test sample, said composition comprising:

(a) about 5 to about 60 millimoles per liter of the composition of a metal-sensitive triphenylmethane dye having the structural formula:
##STR4## wherein X is carbonyl or sulfonyl, n is a number from 0 to about 3, and the R substituents are selected, independently, from the group consisting of hydrogen, hydroxy, an alkyl group including one to about five carbon atoms, bromo, chloro, fluoro, iodo, sulfonate, and phosphate;
(b) a buffer;
(c) a carrier comprising water;
and
(d) about 0.5 to about 4 millimoles per liter of the composition of a chelating agent selected from the group consisting of a polyhydroxy compound, a lignosulfonate, a glucoheptonate, a salicyate complex, a dithionate derivative, a polyethyleneamine, a 2,4-pentanedione, a dipyridine, a polypeptide including cysteine, glycine or histidine, a thiocrown ether, a triphenylphosphine, sorbitol, dimethylglyoxime, proline, bissalicylaldehydeethylenediimine, triethyleneamine, triethylenepyridine amine, 1,4,8,11,22,25-octathiacyclooctosane, ethylenediaminetetraacetic acid, N-(hydroxyethyl) ethylenediaminetriacetic acid, nitrilotriacetic acid, diethylenetriaminepentaacetic acid, aminotris(methylene phosphoric acid), hydroxyethylidene diphosphonic acid, hexamethylenediaminetetra(methylene phosphonate), ethylenediaminediacetic acid, iminodiacetic acid, nitrilopropionic acid, hydroxyethyliminodiacetic acid, pyrophosphoric acid, 1-hydroxyethane-1,1-diphosphonic acid, tripolyphosphoric acid, hexametaphosphoric acid, metaphosphoric acid, and mixtures thereof;
wherein the composition is buffered at a pH of at least about 7.

4 more elements

A method of determining a total divalent cation concentration of an aqueous test sample comprising:

(a) contacting the aqueous test sample with a reagent composition capable of exhibiting a color transition only in response to the total divalent cation concentration of the aqueous test sample, said composition comprising:
(i) about 5 to about 60 millimoles per liter of the composition of a metal-sensitive triphenylmethane dye having the structural formula:
##STR5## wherein X is carbonyl or sulfonyl, n is a number from 0 to about 3, and the R substituents are selected, independently, from the group consisting of hydrogen, hydroxy, an alkyl group including one to about five carbon atoms, bromo, chloro, fluoro, iodo, sulfonate, and phosphate;
(ii) a buffer;
(iii) a carrier comprising water;
and
(iv) about 0.5 to about 4 millimoles per liter of the composition of a chelating agent selected from the group consisting of a polyhydroxy compound, a lignosulfonate, a glucoheptonate, a salicyate complex, a dithionate derivative, a polyethyleneamine, a 2,4-pentanedione, a dipyridine, a polypeptide including cysteine, glycine or histidine, a thiocrown ether, a triphenylphosphine, sorbitol, dimethylglyoxime, proline, bissalicylaldehydeethylenediimine, triethyleneamine, triethylenepyridine amine, 1,4,8,11,22,25-octathiacyclooctosane, ethylenediaminetetraacetic acid, N-(hydroxyethyl)ethylenediaminetriacetic acid, nitrilotriacetic acid, diethylenetriaminepentaacetic acid, aminotris(methylene phosphoric acid), hydroxyethylidene diphosphonic acid, hexamethylenediaminetetra(methylene phosphonate), ethylenediaminediacetic acid, iminodiacetic acid, nitrilopropionic acid, hydroxyethyliminodiacetic acid, pyrophosphoric acid, 1-hydroxyethane-1,1-diphosphonic acid, tripolyphosphoric acid, hexametaphosphoric acid, metaphosphoric acid, and mixtures thereof,
wherein the reagent composition is buffered to a pH of about 7 to about 8;
and
(b) determining the total divalent cation concentration of the aqueous test sample from the intensity and degree of the color transition of the reagent composition.

7 more elements

A method of semiquantitatively determining a specific gravity of an aqueous solution comprising:

(a) contacting the aqueous solution with a reagent composition capable of measuring the total divalent cation concentration of the aqueous solution, said composition comprising:
(i) about 5 to about 60 millimoles per liter of the composition of a metal-sensitive triphenylmethane dye having the structural formula:
##STR6## wherein X is carbonyl or sulfonyl, n is a number from 0 to about 3, and the R substituents are selected, independently, from the group consisting of hydrogen, hydroxy, an alkyl group including one to about five carbon atoms, bromo, chloro, fluoro, iodo, sulfonate, and phosphate;
(ii) a buffer;
(iii) a carrier comprising water;
and
(iv) about 0.5 to about 4 millimoles per liter of the composition of a chelating agent selected from the group consisting of a polyhydroxy compound, a lignosulfonate, a glucoheptonate, a salicyate complex, a dithionate derivative, a polyethyleneamine, a 2,4-pentanedione, a dipyridine, a polypeptide including cysteine, glycine or histidine, a thiocrown ether, a triphenylphosphine, sorbitol, dimethylglyoxime, proline, bissalicylaldehydeethylenediimine, triethyleneamine, triethylenepyridine amine, 1,4,8,11,22,25-octathiacyclooctosane, ethylenediaminetetraacetic acid, N-(hydroxyethyl)ethylenediaminetriacetic acid, nitrilotriacetic acid, diethylenetriaminepentaacetic acid, aminotris(methylene phosphoric acid), hydroxyethylidene diphosphonic acid, hexamethylenediaminetetra(methylene phosphonate), ethylenediaminediacetic acid, iminodiacetic acid, nitrilopropionic acid, hydroxyethyliminodiacetic acid, pyrophosphoric acid, 1-hydroxyethane-1,1-diphosphonic acid, tripolyphosphoric acid, hexametaphosphoric acid, metaphosphoric acid, and mixtures thereof,
wherein the reagent composition is buffered to a pH of about 7;
(b) determining the total divalent cation concentration of the aqueous solution from the intensity and degree of the color transition of the reagent composition, wherein the color transition is in response only to the total divalent cation concentration of the aqueous solution;
and
(c) correlating the total divalent cation concentration of the aqueous solution to the specific gravity of the aqueous solution.

8 more elements

A method of determining a total divalent cation concentration of an aqueous sample comprising:

(a) contacting the aqueous sample with an analyte detection device comprising a test pad, said test pad having incorporated therein a reagent composition capable of exhibiting a color transition only in response to the total divalent cation concentration of the aqueous sample, said composition comprising:
(i) about 5 to about 60 millimoles per liter of the composition of a metal-sensitive triphenylmethane dye having the structural formula:
##STR7## wherein X is carbonyl or sulfonyl, n is a number from 0 to about 3, and the R substituents are selected, independently, from the group consisting of hydrogen, hydroxy, an alkyl group including one to about five carbon atoms, bromo, chloro, fluoro, iodo, sulfonate, and phosphate;
(ii) a buffer;
(iii) a carrier comprising water;
and
(iv) about 0.5 to about 4 millimoles per liter of the composition of a chelating agent selected from the group consisting of a polyhydroxy compound, a lignosulfonate, a glucoheptonate, a salicyate complex, a dithionate derivative, a polyethyleneamine, a 2,4-pentanedione, a dipyridine, a polypeptide including cysteine, glycine or histidine, a thiocrown ether, a triphenylphosphine, sorbitol, dimethylglyoxime, proline, bissalicylaldehydeethylenediimine, triethyleneamine, triethylenepyridine amine, 1,4,8,11,22,25-octathiacyclooctosane, ethylenediaminetetraacetic acid, N-(hydroxyethyl)ethylenediaminetriacetic acid, nitrilotriacetic acid, diethylenetriaminepentaacetic acid, aminotris(methylene phosphoric acid), hydroxyethylidene diphosphonic acid, hexamethylenediaminetetra(methylene phosphonate), ethylenediaminediacetic acid, iminodiacetic acid, nitrilopropionic acid, hydroxyethyliminodiacetic acid, pyrophosphoric acid, 1-hydroxyethane-1,1-diphosphonic acid, tripolyphosphoric acid, hexametaphosphoric acid, metaphosphoric acid, and mixtures thereof,
wherein the reagent composition is buffered to a pH of about 7, and
(b) determining the total divalent cation concentration of the aqueous sample from the intensity and degree of the color transition of the reagent composition.

6 more elements

A method of semiquantitatively determining a specific gravity of an aqueous divalent cation-containing sample comprising:

(a) contacting the aqueous sample with an analyte detection device comprising a test pad having incorporated therein a composition capable of undergoing a color transition only in response to the total divalent cation concentration of the aqueous sample, said composition comprising:
(i) about 5 to about 60 millimoles per liter of the composition of a metal-sensitive triphenylmethane dye having the structural formula:
##STR8## wherein X is carbonyl or sulfonyl, n is a number from 0 to about 3, and the R substituents are selected, independently, from the group consisting of hydrogen, hydroxy, an alkyl group including one to about five carbon atoms, bromo, chloro, fluoro, iodo, sulfonate, and phosphate;
(ii) a buffer;
(iii) a carrier comprising water;
and
(iv) about 0.5 to about 4 millimoles per liter of the composition of a chelating agent selected from the group consisting of a polyhydroxy compound, a lignosulfonate, a glucoheptonate, a salicyate complex, a dithionate derivative, a polyethyleneamine, a 2,4-pentanedione, a dipyridine, a polypeptide including cysteine, glycine or histidine, a thiocrown ether, a triphenylphosphine, sorbitol, dimethylglyoxime, proline, bissalicylaldehydeethylenediimine, triethyleneamine, triethylenepyridine amine, 1,4,8,11,22,25-octathiacyclooctosane, ethylenediaminetetraacetic acid, N-(hydroxyethyl)ethylenediaminetriacetic acid, nitrilotriacetic acid, diethylenetriaminepentaacetic acid, aminotris(methylene phosphoric acid), hydroxyethylidene diphosphonic acid, hexamethylenediaminetetra(methylene phosphonate), ethylenediaminediacetic acid, iminodiacetic acid, nitrilopropionic acid, hydroxyethyliminodiacetic acid, pyrophosphoric acid, 1-hydroxyethane-1,1-diphosphonic acid, tripolyphosphoric acid, hexametaphosphoric acid, metaphosphoric acid, and mixtures thereof,
wherein the reagent composition is buffered to a pH of about 7, and
(b) examining the analyte detection device for a color transition in response only to the total divalent cation content of the aqueous sample;
and
(c) correlating the color transition to the specific gravity of the aqueous sample.

8 more elements

An analyte detection device to determine a total divalent cation concentration of an aqueous test sample comprising:

a support strip;
a test pad;
and
a reagent composition incorporated into the test pad, said reagent composition capable of undergoing a color transition only in response to the total divalent cation concentration of the aqueous sample and said reagent composition comprising:
(a) about 5 to about 60 millimoles per liter of the composition of a metal-sensitive triphenylmethane dye having the structural formula:
##STR9## wherein X is carbonyl or sulfonyl, n is a number from 0 to about 3, and the R substituents are selected, independently, from the group consisting of hydrogen, hydroxy, an alkyl group including one to about five carbon atoms, bromo, chloro, fluoro, iodo, sulfonate, and phosphate;
(b) a buffer;
(c) a carrier comprising water;
and
(d) about 0.5 to about 4 millimoles per liter of the composition of a chelating agent selected from the group consisting of a polyhydroxy compound, a lignosulfonate, a glucoheptonate, a salicyate complex, a dithionate derivative, a polyethyleneamine, a 2,4-pentanedione, a dipyridine, a polypeptide including cysteine, glycine or histidine, a thiocrown ether, a triphenylphosphine, sorbitol, dimethylglyoxime, proline, bissalicylaldehydeethylenediimine, triethyleneamine, triethylenepyridine amine, 1,4,8,11,22,25-octathiacyclooctosane, ethylenediaminetetraacetic acid, N-hydroxyethyl)ethylenediaminetriacetic acid, nitrilotriacetic acid, diethylenetriaminepentaacetic acid, aminotris(methylene phosphoric acid), hydroxyethylidene diphosphonic acid, hexamethylenediaminetetra(methylene phosphonate), ethylenediaminediacetic acid, iminodiacetic acid, nitrilopropionic acid, hydroxyethyliminodiacetic acid, pyrophosphoric acid, 1-hydroxyethane-1,1-diphosphonic acid, tripolyphosphoric acid, hexametaphosphoric acid, metaphosphoric acid, and mixtures thereof,
wherein the reagent composition is buffered at a pH of at least about 7.

8 more elements

Dependencies are inferred from the claim text.

Facts

Application: 08/019,668Filed 19 Feb 1993
Granted: 27 Sep 1994
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

G01N 33/521
Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including…:…
G01N 33/52
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing: use of…
Y10T 436/108331
Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for…
G01N
Investigating or analysing materials by determining their chemical or physical properties

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