Multichamber syringe device for fusing cells

Apparatus for fusing cells comprising:

• (A) a multichamber syringe having a first chamber containing a suspension of cells of a first type, a second chamber containing a suspension of cells of a second type to be added to the suspension of cells of a first type, and a third chamber containing at least 40% by volume polyethylene glycol (PEG) solution, said first, second and third chambers defining respective exit passageways braided together with the downstream ends thereof beveled and facing one another at the same level, the relative cross sections of said chambers being of sufficient dimensions to provide a desired ratio of the suspensions and solution for forming in midair a mixture of 15%-25% PEG by volume;
• (B) a substantially horizontally disposed non-linear tube in fluid communication with said syringe for receiving the mixture therefrom;
• and
• (C) means for causing a reciprocating passage of the mixture through said non-linear tube.