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US 5,350,688 AGranted
Method for regeneration of rice plants
AssigneeKirin Beer Kabushiki Kaisha
InventorsTsukanori Matsuno, Keiichiro Ishizaki
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Abstract
A method is provided for inducing rice callus having embryogenic potency by culturing a rice explant in a liquid medium containing at least inorganic salts, a carbon source, one or more auxins and an osmotic regulator, and for proliferating the callus obtained by subculturing in a similar medium. This method enables the clonal proliferation of rice, so that the invention is useful for the industrial large-scale production of seedlings of all cultivars of rice including non-true bred cultivars and non-true bred lines.
Claims4
A method for producing a rice plant comprising:
- (a) inducing in a liquid medium rice callus having embryogenic potency comprising culturing husked seed of rice (Oryza sativa L.) in a liquid medium substantially free of solidifying agents, said medium containing:
- inorganic salts,
- at least one carbon source,
- at least one auxin, and
- at least one member selected from the group consisting of mannitol, sorbitol and polyethylene glycol;
- (b) proliferating said rice callus of step a) comprising culturing said callus in a liquid medium substantially free of solidifying agents, said medium containing:
- inorganic salts,
- at least one carbon source,
- at least one auxin, and
- at least one member selected from the group consisting of mannitol, sorbitol and polyethylene glycol;
- (c) culturing said callus of step b) in a liquid medium substantially free of solidifying agents, said medium containing:
- inorganic salts,
- at least one carbon source,
- at least one auxin,
- at least one osmotic regulator selected from the group consisting of mannitol, sorbitol and polyethylene glycol,
- at least one cytokinin, and
- at least one member selected from the group consisting of an amino acid and casein hydrolysate,
- the components of said medium provided in amounts sufficient to cause the production of a somatic embryo from said callus;
- and
- (d) culturing said somatic embryo on a semi-solid medium containing:
- inorganic salts,
- at least one carbon source,
- at least one auxin,
- at least one cytokinin,
- at least one member selected from the group consisting of an amino acid and casein hydrolysate, and
- at least one solidifying agent selected from the group consisting of agar and gelrite,
- the components of said medium provided in amounts sufficient to convert said somatic embryo into a rice plant.
24 more elements
A method for producing a rice plant comprising:
- (a) inducing in a liquid medium rice callus having embryogenic potency comprising culturing husked seed of rice (Oryza sativa L.) in a liquid medium substantially free of solidifying agents, said medium containing:
- inorganic salts,
- at least one carbon source,
- at least one auxin, and
- at least one member selected from the group consisting of mannitol, sorbitol and polyethylene glycol;
- (b) proliferating said rice callus of step a) comprising culturing said callus in a liquid medium substantially free of solidifying agents, said medium containing:
- inorganic salts,
- at least one carbon source,
- at least one auxin, and
- at least one member selected from the group consisting of mannitol, sorbitol and polyethylene glycol;
- (c) culturing said callus of step b) in a liquid medium substantially free of solidifying agents, said medium containing:
- inorganic salts,
- at least one carbon source,
- at least one auxin,
- at least one osmotic regulator selected from the group consisting of mannitol, sorbitol and polyethylene glycol,
- at least one cytokinin, abscisic acid, and
- at least one member selected from the group consisting of an amino acid and casein hydrolysate,
- the components of the said medium provided in amounts sufficient to cause the production of a somatic embryo from said callus;
- (d) culturing said somatic embryo of step c) in a liquid medium substantially free of solidifying agents, said medium containing:
- inorganic salts,
- at least one carbon source,
- at least one auxin,
- at least one osmotic regulator selected from the group consisting of mannitol, sorbitol and polyethylene glycol,
- at least one cytokinin,
- at least one member selected from the group consisting of an amino acid and casein hydrolysate,
- the components of said medium provided in amounts sufficient to raise the potency of germination for said somatic embryo;
- and
- (e) culturing said somatic embryo obtained in (d) on a semi-solid medium containing:
- inorganic salts,
- at least one carbon source,
- at least one auxin,
- at least one cytokinin,
- at least one member selected from the group consisting of an amino acid and casein hydrolysate, and
- at least one solidifying agent selected from the group consisting of agar and gelrite,
- the components of said medium provided in amounts sufficient to convert the somatic embryo obtained in (d) into a rice plant.
32 more elements
Dependencies are inferred from the claim text.