Microwave acceleration of enzyme-catalyzed modification of macromolecules
AssigneePioneer Hi-Bred International, Inc.
InventorAnil K. Jhingan
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Abstract
A method of enhancing the enzyme-catalyzed modification of macromolecules comprising exposure of the enzyme and its macromolecule substrate to microwaves is disclosed. A frequency of from 1800 to 3000 MHz and a power of from 50 to 100 watts is preferred. The procedure is rapid, efficient, and suitable for many enzyme-catalyzed reactions. The method can be used for restriction endonuclease digestion of DNA samples and facilitates one of the time-consuming procedures of nucleic acid research. The method is also adaptable for automated procedures in biological research.
Claims15
A method for enzymatic modification of a biological macromolecule comprising the step of exposing a composition comprising the biological macromolecule and an enzyme for which the macromolecule is a substrate to microwaves at a frequency of from 1800 to 3000 MHz and a power of from 50 watts to 1000 watts to accelerate the activity of the enzyme.
Dependencies are inferred from the claim text.