Cephalosporinase testing agent
AssigneeShowa Yakuhin Kako Co., Ltd.
InventorsMatsuhisa Inoue, Akira Sakai
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AssigneeShowa Yakuhin Kako Co., Ltd.
InventorsMatsuhisa Inoue, Akira Sakai
Estimating the term…
A cephalosporinase testing agent comprising a cephalosporin antibiotic, a penicillinase inhibitor in an amount of 20% by weight or less based on the weight of the cephalosporin-antibiotic, and a pH indicator which may be bromcresol purple is provided. A cephalosporinase producing bacteria can be judged by smearing a sample containing bacteria to be tested on the testing agent and observing the color tone change. According to this agent, cephalosporinase-active penicillinase producing bacteria are judged cephalosporinase-negative owing to the action of the penicillinase inhibitor, which may be clavulanic acid. Therefore, since only cephalosporinase producing bacteria are judged cephalosporinase-positive, this agent enables error free judgment.
A cephalosporinase testing agent comprising a cephalosporin antibiotic selected from the group consisting of cefalexin, cefaclor, cefaloglycin, cefadroxil, cefatrizine, cefamandole, cefradine, cefroxadine, cefapirin, cefalotin, cefaloridine, cefotiam, ceftezole, cefazolin and cefacetoril, a penicillinase inhibitor in an amount of 0.5 to 5% by weight based on the weight of the caphalosporin antibiotic, and a pH indicator.
A method for testing a sample from the presence of a cephalosporinase which comprises the steps of (a) smearing a sample comprising bacteria to be tested on a cephalosporinase testing agent comprising a cephalosporin antibiotic selected from the group consisting of cefalexin, cefaclor, cefaloglycin, cefadroxil, cefatrizine, cefamandole, cefradine, cefroxadine, cefapirin, cefalotin, cefaloridine, cefotiam, ceftezole, cefazolin and cefacetoril, a penicillinase inhibitor in an amount of 0.5 to 5% by weight based on the weight of the cephalosporin antibiotic, and a pH indicator, and (b) observing any change of color of the testing agent.
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