Repeat insult microbial test method
AssigneeDow Corning Corporation
InventorCarol A. Hess
Life of this patent
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Abstract
A repeat insult microbial test method in which an antimicrobial agent is applied to a porous permeable substrate. The porous permeable substrate is inoculated with microorganisms, and the porous permeable substrate is incubated for a predetermined period of time, at a temperature which is conducive to the flourishment of the microorganisms. The porous permeable substrate is reinoculated and reincubated a predetermined plurality of times and the inoculated porous permeable substrate is incubated for a final period of time between 18-24 hours at the temperature used previously. The porous permeable substrate is then examined, and the growth of microorganisms is determined.
Claims14
A method for conducting a repeat insult microbial test comprising the steps of (i) applying an antimicrobial agent to a porous permeable substrate selected from the group consisting of collagen film, filter paper, artificial skin, and full thickness human cadaver skin;
- the antimicrobial agent being applied to the porous permeable substrate in a form in which the antimicrobial agent is entrapped as an active ingredient in a nontoxic hydrophobic macroporous highly crosslinked polymer;
- (ii) inoculating the porous permeable substrate with at least one microorganism selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermis, and Escherichia coli;
- (iii) incubating the inoculated porous permeable substrate for a first predetermined period of time at a first temperature which is conducive to the growth and multiplication of the microorganism;
- (iv) repeating the inoculating and incubating steps (ii) and (iii) a predetermined number of times but at least twice;
- (v) incubating the inoculated porous permeable substrate for a final predetermined period of time in excess of the first predetermined period of time at the first temperature;
- the final incubation step (v) being carried out following the last inoculation in step (iv);
- and (vi) examining and determining the amount of growth of microorganisms covering the porous permeable substrate.
A method for conducting a repeat insult microbial test comprising the steps of (i) applying 0.005 to about 0.02 grams of an antimicrobial agent to a porous permeable substrate selected from the group consisting of collagen film, filter paper, artificial skin, and full thickness human cadaver skin;
- the antimicrobial agent being applied to the porous permeable substrate in a form in which the antimicrobial agent is entrapped as an active ingredient in a nontoxic hydrophobic macroporous highly crosslinked polymer;
- (ii) inoculating the porous permeable substrate with at least one microorganism selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermis, and Escherichia coli;
- (iii) incubating the inoculated porous permeable substrate for a first predetermined period of time ranging from five minutes to about four hours at a temperature ranging from 20.degree. to 40.degree. Centigrade;
- (iv) repeating the inoculating and incubating steps (ii) and (iii) a predetermined number of times in the range of two to ten times;
- (v) incubating the inoculated porous permeable substrate for a final predetermined period of time between 18-24 hours at the temperature used in step (iii);
- the final incubation step (v) being carried out following the last inoculation in step (iv);
- and (vi) examining and determining the amount of growth of microorganisms covering the porous permeable substrate.
Dependencies are inferred from the claim text.