Multilayer analytical element for determination of total cholesterol in blood
AssigneeFuji Photo Film Co., Ltd.
InventorsYoshihiko Makino, Kaoru Terashima
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Abstract
In a dry type analytical element having at least one water-permeable layer including a porous spreading layer and at least one water-permeable layer, containing an enzyme having cholesterol esterase activity, the improvement which comprises containing a condensate of poly(ethylene oxide) monoalkylphenyl ether and formaldehyde at least in the water-permeable layer containing the enzyme or another water-permeable layer. In the analytical element of the invention, hemolysis does not occur and bound cholesterol is sufficiently decomposed to free cholesterol.
Claims9
In a dry type integral multilayer analytical element for the quantitative analysis of total cholesterol having at least one hydrophilic polymer layer, one porous reagent layer and one blood cell-separating composite layer for separating blood cells from blood, laminated in this order onto a light-transmissive support, said blood cell-separating composite layer containing a porous spreading layer and containing at least one enzyme having cholesterol esterase activity, cholesterol oxidase and one reagent composition producing a detectable optical change in the presence of hydrogen peroxide, the improvement which comprises said porous reagent layer containing the at least one enzyme having cholesterol esterase activity or the cholesterol oxidase and wherein a part of the at least one hydrophilic layer, the porous reagent layer or the blood cell-separating composite layer contains a condensate represented by the general formula (I):
- ##STR2## wherein m is an integral number of 2 to 4, n is an integral number of not more than 20, and R is an alkyl group having 4 to 12 carbon atoms.
Dependencies are inferred from the claim text.