Back to results
US 5,349,074 AGranted
Process for pharmaceutical grade high purity hyodeoxycholic acid preparation
AssigneeErregierre Industria Chimica S.p.A.
InventorAntonio Bonaldi
Life of this patent
Estimating the term…
Abstract
Process for pharmaceutical grade high purity hyodeoxycholic acid preparation starting from swine bile, consisting in the treatment of swine bile with sodium hydroxide, isolation of hyodeoxycholic, chenodeoxycholic and hyocholic acids by precipiation of their zinc salts and subsequent selective precipitation of the hyodeoxycholic acid magnesium salt, from which the acid is freed by acidification and then crystallized.
Claims14
Process for the preparation of high purity hyodeoxycholic acid starting from swine bile, wherein:
- a) swine bile is made to react with an NaOH aqueous solution;
- b) the reaction mixture is added with an organic solvent plus water and treated with a mineral acid to liberate biliary acids and remove the other bile components;
- c) the biliary acids solution is treated with an ammonium zincate aqueous solution to precipitate the zinc salts of the same biliary acids;
- d) the zinc salts of the biliary acids are treated, in an organic solvent plus water environment, with a mineral acid to liberate the biliary acids;
- e) the biliary acid solution is treated with a magnesium salt to selectively precipitate the magnesium salt of the hyodeoxycholic acid and remove the other biliary acids;
- f) the hyodeoxycholic acid magnesium salt is treated, in an organic solvent plus water environment, with a mineral acid for the purpose of liberating the hyodeoxycholic acid and removing magnesium as a salt of said mineral acid;
- g) hyodeoxycholic acid is recovered at high purity, by crystallization.
4 more elements
Dependencies are inferred from the claim text.