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AssigneeDuke University
InventorsJoseph Bonaventura, Marius Brouwer, Robert E. Cashon
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The invention is directed to a stroma-free tetrameric mammalian hemoglobin which is crosslinked with benzenepentacarboxylate, in which the crosslinking is carried out by a method comprising the step of activating at least two carboxylate groups of the benzenepentacarboxylate with an activating agent prior to reaction with the hemoglobin as well as methods for its production. Crosslinked stroma-free hemoglobin produced by methods of the present invention may be used in applications requiring physiological oxygen carriers such as in blood substitute solutions, or as in a plasma expander.
Stroma-free tetrameric mammalian hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A, and which is crosslinked with benzenepentacarboxylate, in which at least two carboxylate groups have been activated with an activating agent prior to reaction with the hemoglobin.
Stroma-free tetrameric mammalian hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A, and which is crosslinked with benzenepentacarboxylate, in which from about two to three carboxylate groups have been activated with a carbodiimide prior to reaction with the hemoglobin.
A method for producing stroma-free crosslinked hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A, comprising:
A method for producing stroma-free crosslinked hemoglobin having an affinity for oxygen which is lower than that of non-crosslinked hemoglobin A, comprising:
Dependencies are inferred from the claim text.