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US 5,348,963 AGranted
Method of screening for modulators of amyloid formation
AssigneeThe Rockefeller University
InventorsSamuel E. Gandy, Gregg L. Caporaso, Paul Greengard
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Abstract
Agents which modulate or affect the intracellular trafficking and processing of proteins in the mammalian cell can be utilized to affect the trafficking and processing of APP, thereby inhibiting production of Alzheimer type amyloidosis. Particularly useful agents are chloroquine and its related derivatives such as primaquine.
Claims1
An in vitro assay method for screening for agents that modulate amyloid formation which comprises;
- (a) metabolically labeling mammalian cells in culture to produce metabolically labeled mammalian cells;
- (b) contacting the metabolically labeled mammalian cells with an agent suspected of being capable of modulating the trafficking and processing of APP in the mammalian cell during cell metabolism;
- (c) lysing the mammalian cells;
- (d) immunoprecipitating labeled protein of aid cells with an antibody against amyloid precursor protein;
- add
- (e) examining the immunoprecipated APP of said cells for alterations in the trafficking and processing of APP.
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