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AssigneeHBT Holland Biotechnology B.V.
InventorsRemco M. van Es, Gerrit D. Keizer, Albert W. J. van Doorn
Estimating the term…
The invention herein is directed to a method for the visualization of the product of the reaction of a specifically binding substance and a corresponding bindable substance. The reaction product is formed during an assay procedure for the detection and/or determination of one of the components of the reaction. The assay involves the use of a component labeled by a sol of a non-metallic element, such as selenium and tellurium, and the use of a physical developer comprising a metal compound, such as silver lactate and silver nitrate, mercuric chloride and a copper sulfate, to strengthen or reinforce a specific signal, thereby improving detectability.
A method for determining presence or amount of an analyte in a sample, comprising contacting said sample with a binding component capable of specifically recognizing and binding said analyte, said binding component being labelled with colloidal particles of an element selected from the group consisting of selenium and tellurium and determining the presence or absence or the amount of the resulting labelled complexes comprising said analyte, said binding component and said colloidal particles as an indication of the presence or absence of said analyte in said sample and the amount of said resulting labelled complex serving as a measure of the amount of said analyte in said sample, said determination of said labelled complexes consisting of said analyte and said binding component be carried out by physical development of said labelled complex by contacting said labelled complex with a metal compound and a reducing agent capable of liberating the metal from said metal compound in the presence of said colloidal particles for deposition of said metal onto said colloidal particles making up said labelled complex.
A test kit for determining the presence of or the amount of analyte in a test sample comprising a binding component capable of specifically recognizing said analyte, a labelling component comprising colloidal particles of elemental selenium or elemental tellurium for labelling said binding component and a physical developer for resulting formed complex comprising said analyte, said binding component and said colloidal particles, said physical developer comprising a metal compound and a reducing agent capable of liberating metal from said metal compound in the presence of said colloidal particles.
Dependencies are inferred from the claim text.