Method for correcting a calibration curve

A method for determining a corrected measured value of an analyte in a liquid sample utilizing a calibration curve comprising the steps of:

• providing a liquid sample of an analyte;
• analyzing the sample to determine a measured value of the analyte;
• preparing a corrected calibration curve by:
• a) subjecting n calibrators, n is an integer and is greater than 2, containing varying known amounts of the analyte to assay in the same process for the quantitative analysis of the analyte so as to obtain the measured values of said n calibrators, said varying known amounts of analyte being referred to as the calibration values of said calibrator;
• b) determining a linear function of an uncorrected calibration curve using pairs of coordinates of the calibration values and the measured values of said n calibrators excluding a pair of coordinates having a minimum of the calibration value and measured value;
• c) estimating a point on said uncorrected calibration curve, said estimated point having a coordinate of a calibration value and a measured value, the calibration value of said estimated point being selected to be larger than the uppermost value of said calibration values of said n calibrators but not above the upper limit of a determination range for said analyte, the measured value corresponding to said calibration value and being determined by referring to said uncorrected calibration curve;
• d) determining a non-linear function of the corrected calibration curve by plotting said estimated point with the coordinates of said n calibrators;
• comparing the measured value of the analyte to the corrected calibration curve;
• and,
• obtaining a corrected measured value of the analyte.

A method for determining a corrected measured value of an analyte in a liquid sample utilizing a calibration curve comprising the steps of:

• providing a liquid sample of an analyte;
• analyzing the sample to determine a measured value of the analyte;
• preparing a corrected calibration curve by:
• a) subjecting n calibrators, n is an integer and is greater 2, containing varying known amounts of the analyte to assay in the same process for the quantitative analysis of the analyte so as to obtain the measured values of said n calibrators, said varying known amounts of analyte being referred to as the calibration values of said calibrator;
• b) determining a linear function of an uncorrected calibration curve using pairs of coordinates of the calibration values and the measured values of said n calibrators excluding a pair of coordinates having the maximum of the calibration value and measured value;
• c) estimating a point on said uncorrected calibration curve, said estimated point having a coordinate of a calibration value and a measured value, the calibration value of said estimated point being selected to be smaller than the lowermost value of said calibration values of said n calibrators but not below the lower limit of the determination range of said analyte, the measured value corresponding to said calibration value and being determined by referring to said uncorrected calibration curve;
• d) determining a non-linear function of the corrected calibration curve by plotting said estimated point with the coordinates of said calibrators;
• comparing the measured value of the analyte to the corrected calibration curve;
• and,
• obtaining a corrected measured value of the analyte.

A method for determining a corrected measured value of an analyte in a liquid sample utilizing a calibration curve comprising the steps of:

• providing a liquid sample of an analyte;
• analyzing the sample to determine a measured value of the analyte;
• preparing a corrected calibration curve by:
• a) subjecting n calibrators, n is an integer and is greater than 2, containing varying known amounts of the analyte to assay in the same process for the quantitative analysis of the analyte so as to obtain the measured values of said n calibrators, said varying known amounts of analyte being referred to as the calibration values of said calibrator;
• b) determining a linear function of a first uncorrected calibration curve using pairs of coordinates of the calibration values and the measured values of said n calibrators excluding a pair of coordinates having the minimum of the calibration value and measured value;
• c) estimating a first point on said first uncorrected calibration curve, said first point having a coordinate of the first calibration value and first measured value, the first calibration value being selected to be larger than the uppermost value of said calibration values of said n calibrators but not above the upper limit of a determination range for said analyte, the first measured value corresponding to said first calibration value and being determined by referring to said first uncorrected calibration curve;
• d) determining the linear function of a second uncorrected calibration curve using pairs of coordinates of the calibration values and the measured values of said n calibrators excluding a pair of coordinates having the maximum of the calibration value and measured value;
• e) estimating a second point on said second uncorrected calibration curve, said second point having a coordinate of the second calibration value and second measured value, the second calibration value being selected to be smaller than the lowermost value of said calibration values of said n calibrators but not below the lower limit of the determination range of said analyte, the second measured value corresponding to said second calibration value and being determined by referring to said second uncorrected calibration curve;
• f) determining a non-linear function of the corrected calibration curve by plotting said first and second estimated points with the coordinates of said calibrators;
• comparing the measured value of the analyte to the corrected calibration curve;
• and,
• obtaining a corrected measured value of the analyte.