Back to results
US 5,348,877 AGranted
Method of adapting anchorage-dependent cell lines to suspension conditions
AssigneeBoyce Thompson Institute for Plant Research, Inc.
InventorsKevin A. McKenna, Robert R. Granados
Life of this patent
Estimating the term…
Abstract
Normally anchorage-dependent insect cell lines are adapted to replicate under suspension conditions by addition of heparin to the culture medium and selection for resulting suspension-tolerant cells.
Claims14
The method of establishing a suspended cell line from an anchorage-dependent insect cell line comprising the steps of:
- a) providing a culture of normally anchorage-dependent cells in a culture medium,
- b) adding heparin to the culture medium of said anchorage dependent cell line,
- c) selecting the cells which are in suspension after the addition of heparin,
- d) subculturing the cells which were selected in step c) to a fresh culture medium containing heparin,
- e) repeating steps c) and d) until a suspended cell line is established.
2 more elements
A culture of insect cells in suspension wherein the culture medium contains heparin, produced by the steps of:
- a) providing a culture of normally anchorage-dependent cells in a culture medium,
- b) adding heparin to the culture medium of said anchorage dependent cell line,
- c) selecting the cells which are in suspension after the addition of heparin,
- d) subculturing the cells which were selected in step c) to a fresh culture medium containing heparin,
- e) repeating steps c) and d) until a suspended cell line is established.
2 more elements
Dependencies are inferred from the claim text.