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AssigneeInstitute for Molecular Biology & Biotechnology/FORTH
InventorsCharalambos Savakis, Gerald H. Franz, Athanasios Loukeris
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Disclosed are isolated transposable elements, or isolated DNA sequences which encode a transposase protein (or a portion of a transposase protein). The isolated transposable elements or the isolated DNA sequences being characterized by the ability to hybridize to the DNA sequence of Minos 1 under stringent hybridization conditions. The invention also relates to a purified transposase protein, or peptide fragments thereof, encoded by such DNA sequences. Such transposable are useful in methods for the stable introduction of a DNA sequence of interest into a eukaryotic cell. The sequence information disclosed herein is useful in the design of oligonucleotide primers which are useful for the isolation of related members of the Tc-1 family of transposable elements.
An isolated transposable element having a DNA sequence which hybridizes to the DNA sequence of SEQ ID NO:1 in a buffered solution of 0.9 M NaCl, at a temperature of 55.degree. C.
An isolated DNA sequence which encodes a transposase protein, the isolated DNA sequence being characterized by the ability to hybridize to the DNA sequence of SEQ ID NO:1 in a buffered solution of 0.9 M NaCl, at a temperature of 55.degree. C.
A purified transposase protein, encoded by the DNA sequence of SEQ ID NO:2, or DNA sequences which hybridize to the DNA sequence SEQ ID NO:2 in a buffered solution of 0.9 M NaCl, at a temperature of 55.degree. C.
A method for the stable introduction of a DNA sequence of interest into a dipteran insect cell, comprising:
Dependencies are inferred from the claim text.