Immobilization of an anti-thrombogenic substance with a photo-reactive azide and a photo-crosslinking material

A device incorporating an immobilized anti-thrombogenic substance comprising:

• a base coated with a bonding layer comprising a photo-reactive azide derivative macromolecular material soluble in an organic solvent chosen from the group of azide derivative macromolecules consisting of poly-m-azidostyrene, copolymers of poly-m-azidostyrene with styrene, and copolymers of poly-m-azidostyrene with methyl methacrylate which provides covalent bonding when exposed to light;
• and an anti-thrombogenic layer formed by:
• applying an aqueous solution onto the bonding layer coated base to coat the bonding layer with a macromolecular layer, the aqueous solution including a water-soluble photo-crosslinking macromolecular material and the anti-thrombogenic substance;
• irradiating the base with the bonding layer and the macromolecular layer formed thereon with light to fix chemically the macromolecular layer on the base via the bonding layer, the water-soluble photo-crosslinking macromolecular material becoming insoluble and maintaining a gel-condition thereof after being crosslinked by said irradiation.

A method for immobilizing an anti-thrombogenic substance comprising the steps of:

• dissolving a photo-reactive azide derivative macromolecular material, which provides covalent bonding when exposed to light, in an organic solvent to form a bonding solution;
• coating a surface of a base with the bonding solution to form a bonding layer;
• applying an aqueous solution onto the bonding layer coated base to coat the bonding layer with a macromolecular layer, the aqueous solution including a water-soluble photo-crosslinking macromolecular material and the anti-thrombogenic substance, with the water-soluble photo-crosslinking macromolecular material being a material selected from the group consisting of a water-soluble macromolecular material including a photo-reactive group showing a photo-dimeric reaction and a water-soluble macromolecular material having photo-reactive azide group;
• and
• irradiating the base with both the bonding layer and the macromolecular layer formed thereon with light to fix chemically the macromolecular layer on the base via the bonding layer, the water-soluble photo-crosslinking macromolecular material becoming insoluble and maintaining a gel-condition therefor after being crosslinked by said irradiation;
• wherein the anti-thrombogenic substance is retained in the macromolecular layer after said irradiation.