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US 5,348,868 AGranted
Methods and reagents for cleaving and deprotecting oligonucleotides
AssigneeBeckman Instruments, Inc.
InventorsParameswara M. Reddy, Naeem B. Hanna
Life of this patent
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Abstract
Disclosed are reagents of methods for cleaving and deprotecting insolubilized and protected synthetic oligonucleotides. In a particularly preferred embodiment, the reagent comprises methylamine and t-butylamine.
Claims14
A method for cleaving a support-bound oligonucleotide and/or removing at least one protecting group from a protected oligonucleotide, the method comprising the steps of:
- a) introducing an oligonucleotide selected from the group consisting of
- i) at least one insoluble, protected oligonucleotide;
- ii) at least one soluble, protected oligonucleotide;
- and
- iii) at least one insoluble oligonucleotide,
- to a reagent to form a mixture, said reagent comprising methylamine;
- and
- b) incubating said mixture for a sufficient time period at a sufficient temperature to obtain at least one biologically useful oligonucleotide.
5 more elements
A method for cleaving a support-bound oligonucleotide and/or removing at least one protecting group from a protected oligonucleotide, the method comprising the steps of:
- a) introducing an oligonucleotide selected from the group consisting of
- i) at least one insoluble, protected oligonucleotide;
- ii) at least one soluble, protected oligonucleotide;
- and
- iii) at least one insoluble oligonucleotide to a reagent to form a mixture, said reagent comprising methylamine and aqueous ammonia;
- and
- b) incubating said mixture for a sufficient time period at a sufficient temperature to obtain at least one soluble oligonucleotide.
4 more elements
Dependencies are inferred from the claim text.