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US 5,348,860 AGranted
Screening test and kit for cancerous and precancerous conditions
OwnerAbulkalam M. Shamsuddininventor
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OwnerAbulkalam M. Shamsuddininventor
Estimating the term…
An improved screening test method and kit for cancerous and precancerous conditions which is rapid, employs reagents which can be provided in kit form and which does not give false negatives due to sampling error, immobilizes a sample of a proteinaceous body fluid in a membrane filter, tests for marker carbohydrates therein which have vicinal galactose moieties and which are enzymatically oxidized with galactose oxidase to vicinal aldehydic moieties which then visualizes any thus-produced vicinal aldehydic moieties with Schiff's Reagent, then further oxidizes those samples which test negative for the marker carbohydrates with periodic acid, thereby rendering the sample visualizable with Schiff's Reagent if the sample contains glycoprotein, which applies the galactose oxidase directly to the membrane filter, thereby speeding up color developed, and employs Schiff's Reagent which was refrigerated after preparation until its color faded to a straw shade before being treated with activated charcoal, which treatment renders the solution storage-stable for many months and enhances its color development ability.
A screening method for rapidly testing a human being for a cancerous or precancerous condition and as part of the same testing eliminating the possibility of a false negative, whereby the individual can be immediately retested if a negative assay is due to procedural error, which comprises the steps of (a) obtaining a sample of rectal mucus from an individual;
A method of eliminating a false negative as a result of mucus sampling error in a rectal mucus method for screening for a cancerous or precancerous condition in a human being, which comprises conducting, within about a half hour period after collecting a sample of rectal mucus, the steps of (a) subjecting a first portion of the sample of rectal mucus in an assay for the presence therein of at least one marker carbohydrate selected from the group consisting of beta-D-Gal-(1->3)-D-GalNAc, Fuc-alpha-1->2 Gal-Beta(1->4)-Fuc-alpha-1->3-GlcNAc, Fuc-Alpha-1->2Gal-beta-(1->4)-Fuc-alpha-1->3-GlcNAc-beta-(1->3)-Gal-beta-( 1>4)-GlcNAc and Fuc-alpha-1->2-Gal-beta-(1->4)-Fuc-alpha-1->3-GlcNA-beta-(1->3)-Gal-beta-( 1->4)-Fuc-alpha-1->3-GlcNAc, and (b) either concurrently subjecting another portion of the same mucus sample or, if the first portion tests negative in step (a), thereafter subjecting the same portion, to oxidizing conditions which are capable of oxidative ring opening of the cyclic sugar moieties of any glycoprotein present in the sample at a hydroxy group-bearing ring carbon atom thereof to form aldehydic sugar moieties and then assaying the thus-oxidized sample for the presence therein of any aldehydic sugar moieties thus formed, the presence of thereby formed aldehydic sugar moieties confirming the adequacy of the mucus sampling and additionally, when the assay for marker carbohydrates is negative, that none of the marker carbohydrates are present in the mucus sample.
In a process for producing an aqueous solution of basic fuchsin wherein a basic solution of fuchsin and sodium bisulfate is treated with activated charcoal and is stored at below room temperature, the improvement which comprises storing the basic solution at below room temperature until the color thereof fades to a straw color before the activated charcoal is added thereto, thereby rendering the solution storage-stable and enhancing its color development ability.
A method for screening for a cancerous or precancerous condition in an organ other than the large intestine of a human being, which comprises subjecting a sample of proteinaceous secretion other than rectal mucus associated with that organ, to an assay for the presence therein of a marker carbohydrate selected from the group consisting of beta-D-Gal-(1->3)-D-GalNAc, Fuc-alpha-1->2 Gal-Beta(1->4)-Fuc-alpha-1->3-GlcNAc, Fuc-Alpha-1->2 Gal-beta-(1->4)-Fuc-alpha-1-22 3-GlcNAc-beta-(1->3)-Gal-beta-(1>4)-GlcNAc and Fuc-alpha-1->2-Gal-beta-(1->4)-Fuc-alpha-1->3-GlcNAc-beta-(1->3)Gal-beta-( 1->4)-Fuc-alpha-1->3-GlcNAc.
Dependencies are inferred from the claim text.